Show simple item record

dc.contributor.authorZwar, TA
dc.contributor.authorRead, S
dc.contributor.authorvan Driel, IR
dc.contributor.authorGleeson, PA
dc.identifierpii: 176/3/1609
dc.identifier.citationZwar, T. A., Read, S., van Driel, I. R. & Gleeson, P. A. (2006). CD4(+)CD25(+) regulatory T cells inhibit the antigen-dependent expansion of self-reactive T cells in vivo. JOURNAL OF IMMUNOLOGY, 176 (3), pp.1609-1617.
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractA deficiency of CD4+CD25+ regulatory T cells (CD25+ Tregs) in lymphopenic mice can result in the onset of autoimmune gastritis. The gastric H/K ATPase alpha (H/Kalpha) and beta (H/Kbeta) subunits are the immunodominant autoantigens recognized by effector CD4+ T cells in autoimmune gastritis. The mechanism by which CD25+ Tregs suppress autoimmune gastritis in lymphopenic mice is poorly understood. To investigate the antigenic requirements for the genesis and survival of gastritis-protecting CD25+ Tregs, we analyzed mice deficient in H/Kbeta and H/Kalpha, as well as a transgenic mouse line (H/Kbeta-tsA58 Tg line 224) that lacks differentiated gastric epithelial cells. By adoptive transfer of purified T cell populations to athymic mice, we show that the CD25+ Treg population from mice deficient in either one or both of H/Kalpha and H/Kbeta, or from the H/Kbeta-tsA58 Tg line 224 mice, is equally effective in suppressing the ability of polyclonal populations of effector CD4+ T cells to induce autoimmune gastritis. Furthermore, CD25+ Tregs, from either wild-type or H/Kalpha-deficient mice, dramatically reduced the expansion of pathogenic H/Kalpha-specific TCR transgenic T cells and the induction of autoimmune gastritis in athymic recipient mice. Proliferation of H/Kalpha-specific T cells in lymphopenic hosts occurs predominantly in the paragastric lymph node and was dependent on the presence of the cognate H/Kalpha Ag. Collectively, these studies demonstrate that the gastritis-protecting CD25+ Tregs do not depend on the major gastric Ags for their thymic development or their survival in the periphery, and that CD25+ Tregs inhibit the Ag-specific expansion of pathogenic T cells in vivo.
dc.subjectBiochemistry and Cell Biology not elsewhere classified ; Biological Sciences
dc.titleCD4(+)CD25(+) regulatory T cells inhibit the antigen-dependent expansion of self-reactive T cells in vivo
dc.typeJournal Article
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentBiochemistry And Molecular Biology
melbourne.source.titleJOURNAL OF IMMUNOLOGY
melbourne.contributor.authorvan Driel, Ian
melbourne.contributor.authorGleeson, Paul
melbourne.contributor.authorZWAR, TRICIA
melbourne.contributor.authorREAD, SIMON
melbourne.accessrightsThis item is currently not available from this repository

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record