Increased survival of auditory neurones treated with LIF
AuthorMarzella, P. L.; Clark, Graeme M.; Shepherd, R. K.; Bartlett, P. F.; Kilpatrick, T. J.
Source TitleProceedings of the Australian Neuroscience Society
University of Melbourne Author/sClark, Graeme; MARZELLA, PHILLIP; Shepherd, Robert; Kilpatrick, Trevor
Document TypeConference Item
CitationsMarzella, P. L., Clark, G. M., Shepherd, R. K., Bartlett, P. F., & Kilpatrick, T. J. (1997). Increased survival of auditory neurones treated with LIF. In Proceedings of the Australian Neuroscience Society, Newcastle.
Access StatusOpen Access
This is an abstract of a paper from Proceedings of the Australian Neuroscience Society 1997 published by Australian Neuroscience Society. This version is reproduced with the permission of publisher.
Degeneration of spiral ganglion cells (SOC) is one of the most common correlates of sensorineural hearing loss (1). Several lines of evidence show that the continued supply of growth factors is responsible for maintaining auditory neurone integrity (2). In the present study SOC cultures were used as a model of auditory innervation to test the ability of the cytokine leukaemia inhibitory factor (LIF) and the neurotrophin NT -3 to promote neuronal survival individually or in combination. The data demonstrate that LIF promotes the survival of SOC in a concentration-dependent manner, with a significant increase in neuronal survival at concentrations as low as 0.1 ng/ml compared to untreated wells ( p< 0.05), and a maximum neuronal survival at 10 ng/ml. In addition, when used in combination LIF and NT-3 were more effective in promoting neuronal survival than either factor individually, with a significant increase in survival at concentrations of 0.1ng mI[to the power of]-1/0.1 ng mI[to the power of]-1 (LIF/NT-3). To our knowledge this is the first study reporting that LIF has trophic activity on SOC. Moreover, the data suggest that a combination of several growth factors may provide a better approach when developing pharmacological therapies for auditory neuron repair.
Keywordsotolaryngology; otology; cochlear implants; spiral ganglion cells; neuronal survival
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