The effect of polypyrrole with incorporated neurotrophin-3 on the promotion of neurite outgrowth from auditory neurons
AuthorRichardson, RT; Thompson, B; Moulton, S; Newbold, C; Lum, MG; Cameron, A; Wallace, G; Kapsa, R; Clark, G; O'Leary, S
PublisherELSEVIER SCI LTD
University of Melbourne Author/sRichardson, Rachael; NEWBOLD, CARRIE JEAN; O'Leary, Stephen; Clark, Graeme
Document TypeJournal Article
CitationsRichardson, R. T., Thompson, B., Moulton, S., Newbold, C., Lum, M. G., Cameron, A., Wallace, G., Kapsa, R., Clark, G. & O'Leary, S. (2007). The effect of polypyrrole with incorporated neurotrophin-3 on the promotion of neurite outgrowth from auditory neurons. BIOMATERIALS, 28 (3), pp.513-523. https://doi.org/10.1016/j.biomaterials.2006.09.008.
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This research aims to improve the nerve-electrode interface of the cochlear implant using polymer technology to encourage neuron survival, elongation and adhesion to the electrodes. Polypyrrole (Ppy) doped with p-toluene sulphonate (pTS) is an electroactive polymer into which neurotrophin-3 (NT3) can be incorporated. Ppy/pTS+/-NT3 was synthesised over gold electrodes and used as a surface for auditory neuron explant culture. Neurite outgrowth from explants grown on Ppy/pTS was equivalent to tissue culture plastic but improved with the incorporation of NT3 (Ppy/pTS/NT3). Electrical stimulation of Ppy/pTS/NT3 with a biphasic current pulse, as used in cochlear implants, significantly improved neurite outgrowth from explants. Using (125)I-NT3, it was shown that low levels of NT3 passively diffused from Ppy/pTS/NT3 during normal incubation and that electrical stimulation enhanced the release of biologically active NT3 in quantities adequate for neuron survival. Furthermore, Ppy/pTS/NT3 and its constituents were not toxic to auditory neurons and the Ppy/pTS/NT3 coating on gold electrodes did not alter impedance. If applied to the cochlear implant, Ppy/pTS/NT3 will provide a biocompatible, low-impedance substrate for storage and release of NT3 to help protect auditory neurons from degradation after sensorineural hearing loss and encourage neurite outgrowth towards the electrodes.
Keywordscontrolled drug release; electroactive polymer; electrical stimulation; nerve growth factor; nerve regeneration
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