Murine hematopoietic blast colony-forming cells and their progeny have distinctive membrane marker profiles
AuthorMetcalf, D; Ng, AP; Loughran, SJL; Phipson, B; Smyth, GK; Di Rago, L; Mifsud, S
Source TitlePROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
PublisherNATL ACAD SCIENCES
AffiliationFaculty of Medicine, Dentistry & Health Sciences, Medical Biology (W.E.H.I.)
Document TypeJournal Article
CitationsMetcalf, D; Ng, AP; Loughran, SJL; Phipson, B; Smyth, GK; Di Rago, L; Mifsud, S, Murine hematopoietic blast colony-forming cells and their progeny have distinctive membrane marker profiles, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (45), pp. 19102 - 19107
Access StatusAccess this item via the Open Access location
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC2776469
© 2009 The Authors
Two distinct bone marrow-derived blast colony-forming cells can generate colonies of lineage-restricted progenitor cells in agar cultures of murine bone marrow. Both cell types selectively had a Kit(+) ScaI(+) phenotype distinguishing them from most lineage-restricted progenitor cells. Multicentric blast colony-forming cells stimulated by stem cell factor plus interleukin-6 (IL-6) (BL-CFC-S) were separable from most dispersed blast colony-forming cells stimulated by Flt3 ligand and IL-6 (BL-CFC-F) using CD34 and Flt3R probes. Multicentric BL-CFC-S cofractionated with colony-forming units, spleen (CFU-S) supporting the possibility that the 2 cells may be identical. The colony populations generated by BL-CFC-S were similar in their phenotype and proliferative capacity to progenitor cells in whole bone marrow but the progeny of BL-CFC-F were skewed with an abnormally high proportion of Kit(-) Flt3R(+) cells whose clonogenic cells tended to generate only macrophage progeny. Both blast colony populations had a high percentage of GR1(+) and Mac1(+) cells but BL-CFC-F colonies also contained a significant population of B220(+) and IL-7R(+) cells relevant to the superior ability of BL-CFC-F colony cells to generate B lymphocytes and the known dependency of this process on Flt3 ligand and IL-7. The commitment events and phenotypic changes during the generation of differing progenitor cells in blast colonies can now be clonally analyzed in a convenient in vitro culture system.
Keywordsblast colonies; lineage commitment; hematopoietic stem cells
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