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dc.contributor.authorLinklater, DP
dc.contributor.authorBaulin, VA
dc.contributor.authorLe Guevel, X
dc.contributor.authorFleury, J-B
dc.contributor.authorHanssen, E
dc.contributor.authorNguyen, THP
dc.contributor.authorJuodkazis, S
dc.contributor.authorBryant, G
dc.contributor.authorCrawford, RJ
dc.contributor.authorStoodley, P
dc.contributor.authorIvanova, EP
dc.date.accessioned2021-07-01T01:07:00Z
dc.date.available2021-07-01T01:07:00Z
dc.date.issued2020-12-28
dc.identifier.citationLinklater, D. P., Baulin, V. A., Le Guevel, X., Fleury, J. -B., Hanssen, E., Nguyen, T. H. P., Juodkazis, S., Bryant, G., Crawford, R. J., Stoodley, P. & Ivanova, E. P. (2020). Antibacterial Action of Nanoparticles by Lethal Stretching of Bacterial Cell Membranes. ADVANCED MATERIALS, 32 (52), https://doi.org/10.1002/adma.202005679.
dc.identifier.issn0935-9648
dc.identifier.urihttp://hdl.handle.net/11343/276603
dc.description.abstractIt is commonly accepted that nanoparticles (NPs) can kill bacteria; however, the mechanism of antimicrobial action remains obscure for large NPs that cannot translocate the bacterial cell wall. It is demonstrated that the increase in membrane tension caused by the adsorption of NPs is responsible for mechanical deformation, leading to cell rupture and death. A biophysical model of the NP–membrane interactions is presented which suggests that adsorbed NPs cause membrane stretching and squeezing. This general phenomenon is demonstrated experimentally using both model membranes and Pseudomonas aeruginosa and Staphylococcus aureus, representing Gram‐positive and Gram‐negative bacteria. Hydrophilic and hydrophobic quasi‐spherical and star‐shaped gold (Au)NPs are synthesized to explore the antibacterial mechanism of non‐translocating AuNPs. Direct observation of nanoparticle‐induced membrane tension and squeezing is demonstrated using a custom‐designed microfluidic device, which relieves contraction of the model membrane surface area and eventual lipid bilayer collapse. Quasi‐spherical nanoparticles exhibit a greater bactericidal action due to a higher interactive affinity, resulting in greater membrane stretching and rupturing, corroborating the theoretical model. Electron microscopy techniques are used to characterize the NP–bacterial‐membrane interactions. This combination of experimental and theoretical results confirm the proposed mechanism of membrane‐tension‐induced (mechanical) killing of bacterial cells by non‐translocating NPs.
dc.languageEnglish
dc.publisherWILEY-V C H VERLAG GMBH
dc.titleAntibacterial Action of Nanoparticles by Lethal Stretching of Bacterial Cell Membranes
dc.typeJournal Article
dc.identifier.doi10.1002/adma.202005679
melbourne.affiliation.departmentBio21
melbourne.affiliation.facultyAffiliate
melbourne.source.titleAdvanced Materials
melbourne.source.volume32
melbourne.source.issue52
melbourne.elementsid1479581
melbourne.internal.embargodate2021-11-12
melbourne.contributor.authorHanssen, Eric
dc.identifier.eissn1521-4095
melbourne.accessrightsThis item is currently not available from this repository


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