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dc.contributor.authorKoernig, S
dc.contributor.authorCampbell, IK
dc.contributor.authorMackenzie-Kludas, C
dc.contributor.authorSchaub, A
dc.contributor.authorLoetscher, M
dc.contributor.authorNg, WC
dc.contributor.authorZehnder, R
dc.contributor.authorPelczar, P
dc.contributor.authorSanli, I
dc.contributor.authorAlhamdoosh, M
dc.contributor.authorNg, M
dc.contributor.authorBrown, LE
dc.contributor.authorKasermann, F
dc.contributor.authorVonarburg, C
dc.contributor.authorZuercher, AW
dc.date.accessioned2021-07-13T00:23:00Z
dc.date.available2021-07-13T00:23:00Z
dc.date.issued2019-07-01
dc.identifierpii: 10.1038/s41385-019-0167-z
dc.identifier.citationKoernig, S., Campbell, I. K., Mackenzie-Kludas, C., Schaub, A., Loetscher, M., Ng, W. C., Zehnder, R., Pelczar, P., Sanli, I., Alhamdoosh, M., Ng, M., Brown, L. E., Kasermann, F., Vonarburg, C. & Zuercher, A. W. (2019). Topical application of human-derived Ig isotypes for the control of acute respiratory infection evaluated in a human CD89-expressing mouse model. MUCOSAL IMMUNOLOGY, 12 (4), pp.1013-1024. https://doi.org/10.1038/s41385-019-0167-z.
dc.identifier.issn1933-0219
dc.identifier.urihttp://hdl.handle.net/11343/277806
dc.description.abstractRecurrent and persistent airway infections remain prevalent in patients with primary immunodeficiency (PID), despite restoration of serum immunoglobulin levels by intravenous or subcutaneous plasma-derived IgG. We investigated the effectiveness of different human Ig isotype preparations to protect mice against influenza when delivered directly to the respiratory mucosa. Four polyvalent Ig preparations from pooled plasma were compared: IgG, monomeric IgA (mIgA), polymeric IgA-containing IgM (IgAM) and IgAM associated with the secretory component (SIgAM). To evaluate these preparations, a transgenic mouse expressing human FcαRI/CD89 within the myeloid lineage was created. CD89 was expressed on all myeloid cells in the lung and blood except eosinophils, reflecting human CD89 expression. Intranasal administration of IgA-containing preparations was less effective than IgG in reducing pulmonary viral titres after infection of mice with A/California/7/09 (Cal7) or the antigenically distant A/Puerto Rico/8/34 (PR8) viruses. However, IgA reduced weight loss and inflammatory mediator expression. Both IgG and IgA protected mice from a lethal dose of PR8 virus and for mIgA, this effect was partially CD89 dependent. Our data support the beneficial effect of topically applied Ig purified from pooled human plasma for controlling circulating and non-circulating influenza virus infections. This may be important for reducing morbidity in PID patients.
dc.languageEnglish
dc.publisherNATURE PUBLISHING GROUP
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleTopical application of human-derived Ig isotypes for the control of acute respiratory infection evaluated in a human CD89-expressing mouse model
dc.typeJournal Article
dc.identifier.doi10.1038/s41385-019-0167-z
melbourne.affiliation.departmentSir Peter MacCallum Department of Oncology
melbourne.affiliation.departmentMicrobiology and Immunology
melbourne.affiliation.departmentBio21
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.affiliation.facultyAffiliate
melbourne.source.titleMucosal Immunology
melbourne.source.volume12
melbourne.source.issue4
melbourne.source.pages1013-1024
dc.rights.licenseCC BY
melbourne.elementsid1397230
melbourne.contributor.authorMackenzie-Kludas, Charley
melbourne.contributor.authorBrown, Lorena
melbourne.contributor.authorAlhamdoosh, Monther
melbourne.contributor.authorCampbell, Ian
melbourne.contributor.authorNg, Milica
dc.identifier.eissn1935-3456
melbourne.accessrightsOpen Access


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