CD8(+) T cells specific for an immunodominant SARS-CoV-2 nucleocapsid epitope display high naive precursor frequency and TCR promiscuity
AuthorNguyen, THO; Rowntree, LC; Petersen, J; Chua, BY; Hensen, L; Kedzierski, L; van de Sandt, CE; Chaurasia, P; Tan, H-X; Habel, JR; ...
University of Melbourne Author/sMordant, Francesca; Rowntree, Louise; Hensen, Luca; Kedzierski, Lukasz; Kent, Stephen; Kedzierska, Katherine; Wakim, Linda; Torresi, Joseph; Wheatley, Adam; Nguyen, Thi Hoang Oanh; ...
AffiliationMicrobiology and Immunology
Veterinary and Agricultural Sciences
Document TypeJournal Article
CitationsNguyen, T. H. O., Rowntree, L. C., Petersen, J., Chua, B. Y., Hensen, L., Kedzierski, L., van de Sandt, C. E., Chaurasia, P., Tan, H. -X., Habel, J. R., Zhang, W., Allen, L. F., Earnest, L., Mak, K. Y., Juno, J. A., Wragg, K., Mordant, F. L., Amanat, F., Krammer, F. ,... Kedzierska, K. (2021). CD8(+) T cells specific for an immunodominant SARS-CoV-2 nucleocapsid epitope display high naive precursor frequency and TCR promiscuity. IMMUNITY, 54 (5), pp.1066-+. https://doi.org/10.1016/j.immuni.2021.04.009.
Access StatusAccess this item via the Open Access location
Open Access URLPublished version
NHMRC Grant codeNHMRC/1123673
To better understand primary and recall T cell responses during coronavirus disease 2019 (COVID-19), it is important to examine unmanipulated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-specific T cells. By using peptide-human leukocyte antigen (HLA) tetramers for direct ex vivo analysis, we characterized CD8+ T cells specific for SARS-CoV-2 epitopes in COVID-19 patients and unexposed individuals. Unlike CD8+ T cells directed toward subdominant epitopes (B7/N257, A2/S269, and A24/S1,208) CD8+ T cells specific for the immunodominant B7/N105 epitope were detected at high frequencies in pre-pandemic samples and at increased frequencies during acute COVID-19 and convalescence. SARS-CoV-2-specific CD8+ T cells in pre-pandemic samples from children, adults, and elderly individuals predominantly displayed a naive phenotype, indicating a lack of previous cross-reactive exposures. T cell receptor (TCR) analyses revealed diverse TCRαβ repertoires and promiscuous αβ-TCR pairing within B7/N105+CD8+ T cells. Our study demonstrates high naive precursor frequency and TCRαβ diversity within immunodominant B7/N105-specific CD8+ T cells and provides insight into SARS-CoV-2-specific T cell origins and subsequent responses.
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