Regulation of interleukin 1 alpha, activin and inhibin by lipopolysaccharide in Sertoli cells from prepubertal rats
AuthorWinnall, WR; Okuma, Y; Saito, K; Muir, JA; Hedger, MP
Source TitleMolecular and Cellular Endocrinology
PublisherELSEVIER IRELAND LTD
University of Melbourne Author/sWINNALL, WENDY
AffiliationMicrobiology And Immunology
Document TypeJournal Article
CitationsWinnall, W. R., Okuma, Y., Saito, K., Muir, J. A. & Hedger, M. P. (2009). Regulation of interleukin 1 alpha, activin and inhibin by lipopolysaccharide in Sertoli cells from prepubertal rats. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 307 (1-2), pp.169-175. https://doi.org/10.1016/j.mce.2009.02.007.
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C1 - Journal Articles Refereed
Bacterial lipopolysaccharide increased the production of interleukin 1alpha and activin A, and reduced production of inhibin B, in Sertoli cells from immature male rats measured by enzyme-linked immunosorbent assay (ELISA). The majority of immunoreactive interleukin 1alpha remained within the Sertoli cell, while both activin A and inhibin B were secreted. Lipopolysaccharide-stimulated expression of two interleukin 1alpha mRNA transcripts, measured by quantitative RT-PCR, but the levels of bioactive interleukin 1alpha in Sertoli cell extracts and medium, measured by in vitro bioassay, were comparatively low to undetectable. A specific antagonist of interleukin 1alpha had no effect on lipopolysaccharide-stimulated activin A or inhibin B responses. These data indicate that, in contrast to Sertoli cells from adult rats, lipopolysaccharide-induced regulation of activin A and inhibin B by prepubertal Sertoli cells does not involve secreted interleukin 1alpha. The data highlight the possibility of a role for intracellular interleukin 1alpha in the Sertoli cell response to inflammation, particularly in the immature testis.
KeywordsInnate Immunity; Reproduction; Immune System and Allergy; Reproductive System and Disorders
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