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dc.contributor.authorWinnall, WR
dc.contributor.authorOkuma, Y
dc.contributor.authorSaito, K
dc.contributor.authorMuir, JA
dc.contributor.authorHedger, MP
dc.identifierpii: S0303-7207(09)00130-0
dc.identifier.citationWinnall, W. R., Okuma, Y., Saito, K., Muir, J. A. & Hedger, M. P. (2009). Regulation of interleukin 1 alpha, activin and inhibin by lipopolysaccharide in Sertoli cells from prepubertal rats. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 307 (1-2), pp.169-175.
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractBacterial lipopolysaccharide increased the production of interleukin 1alpha and activin A, and reduced production of inhibin B, in Sertoli cells from immature male rats measured by enzyme-linked immunosorbent assay (ELISA). The majority of immunoreactive interleukin 1alpha remained within the Sertoli cell, while both activin A and inhibin B were secreted. Lipopolysaccharide-stimulated expression of two interleukin 1alpha mRNA transcripts, measured by quantitative RT-PCR, but the levels of bioactive interleukin 1alpha in Sertoli cell extracts and medium, measured by in vitro bioassay, were comparatively low to undetectable. A specific antagonist of interleukin 1alpha had no effect on lipopolysaccharide-stimulated activin A or inhibin B responses. These data indicate that, in contrast to Sertoli cells from adult rats, lipopolysaccharide-induced regulation of activin A and inhibin B by prepubertal Sertoli cells does not involve secreted interleukin 1alpha. The data highlight the possibility of a role for intracellular interleukin 1alpha in the Sertoli cell response to inflammation, particularly in the immature testis.
dc.subjectInnate Immunity; Reproduction; Immune System and Allergy; Reproductive System and Disorders
dc.titleRegulation of interleukin 1 alpha, activin and inhibin by lipopolysaccharide in Sertoli cells from prepubertal rats
dc.typeJournal Article
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentMicrobiology And Immunology
melbourne.source.titleMolecular and Cellular Endocrinology
melbourne.contributor.authorWINNALL, WENDY
melbourne.fieldofresearch320407 Innate immunity
melbourne.fieldofresearch321503 Reproduction
melbourne.seocode200199 Clinical health not elsewhere classified
melbourne.accessrightsThis item is currently not available from this repository

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