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dc.contributor.authorWinnall, WR
dc.contributor.authorOkuma, Y
dc.contributor.authorSaito, K
dc.contributor.authorMuir, JA
dc.contributor.authorHedger, MP
dc.date.available2014-05-21T22:21:30Z
dc.date.available2009-02-16
dc.date.issued2009-08-13
dc.identifierpii: S0303-7207(09)00130-0
dc.identifier.citationWinnall, W. R., Okuma, Y., Saito, K., Muir, J. A. & Hedger, M. P. (2009). Regulation of interleukin 1 alpha, activin and inhibin by lipopolysaccharide in Sertoli cells from prepubertal rats. MOLECULAR AND CELLULAR ENDOCRINOLOGY, 307 (1-2), pp.169-175. https://doi.org/10.1016/j.mce.2009.02.007.
dc.identifier.issn0303-7207
dc.identifier.urihttp://hdl.handle.net/11343/28984
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractBacterial lipopolysaccharide increased the production of interleukin 1alpha and activin A, and reduced production of inhibin B, in Sertoli cells from immature male rats measured by enzyme-linked immunosorbent assay (ELISA). The majority of immunoreactive interleukin 1alpha remained within the Sertoli cell, while both activin A and inhibin B were secreted. Lipopolysaccharide-stimulated expression of two interleukin 1alpha mRNA transcripts, measured by quantitative RT-PCR, but the levels of bioactive interleukin 1alpha in Sertoli cell extracts and medium, measured by in vitro bioassay, were comparatively low to undetectable. A specific antagonist of interleukin 1alpha had no effect on lipopolysaccharide-stimulated activin A or inhibin B responses. These data indicate that, in contrast to Sertoli cells from adult rats, lipopolysaccharide-induced regulation of activin A and inhibin B by prepubertal Sertoli cells does not involve secreted interleukin 1alpha. The data highlight the possibility of a role for intracellular interleukin 1alpha in the Sertoli cell response to inflammation, particularly in the immature testis.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherELSEVIER IRELAND LTD
dc.subjectInnate Immunity; Reproduction; Immune System and Allergy; Reproductive System and Disorders
dc.titleRegulation of interleukin 1 alpha, activin and inhibin by lipopolysaccharide in Sertoli cells from prepubertal rats
dc.typeJournal Article
dc.identifier.doi10.1016/j.mce.2009.02.007
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentMicrobiology And Immunology
melbourne.source.titleMolecular and Cellular Endocrinology
melbourne.source.volume307
melbourne.source.issue1-2
melbourne.source.pages169-175
melbourne.publicationid160981
melbourne.elementsid333232
melbourne.contributor.authorWINNALL, WENDY
dc.identifier.eissn1872-8057
melbourne.fieldofresearch320407 Innate immunity
melbourne.fieldofresearch321503 Reproduction
melbourne.seocode200199 Clinical health not elsewhere classified
melbourne.accessrightsThis item is currently not available from this repository


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