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    The effect of selective oestrogen receptor antagonists in an in vitro model of growth plate chondrogenesis

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    Author
    Simm, PJ; Russo, VC; Werther, GA
    Date
    2011-08-01
    Source Title
    ENDOCRINE
    Publisher
    HUMANA PRESS INC
    University of Melbourne Author/s
    Russo, Vincent; Werther, George; Simm, Peter
    Affiliation
    Paediatrics Royal Children'S Hospital
    Metadata
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    Document Type
    Journal Article
    Citations
    Simm, P. J., Russo, V. C. & Werther, G. A. (2011). The effect of selective oestrogen receptor antagonists in an in vitro model of growth plate chondrogenesis. ENDOCRINE, 40 (1), pp.27-34. https://doi.org/10.1007/s12020-011-9473-2.
    Access Status
    This item is currently not available from this repository
    URI
    http://hdl.handle.net/11343/29072
    DOI
    10.1007/s12020-011-9473-2
    Description

    C1 - Journal Articles Refereed

    Abstract
    While oestrogen is recognized to play a key role in regulating growth, particularly in relation to epiphyseal fusion, the mechanisms that mediate its effects are still unclear. We utilized an in vitro model of chondrogenesis, the RCJ3.1C5.18 cell line, to explore the effect of oestrogen on this process. We demonstrated the presence of oestrogen receptors (ER) α and β in these cells, with increased abundance of both receptor sub-types evident as the cells differentiated. ERα localized to the nucleus, suggesting it was signalling by genomic pathways, while ERβ was seen predominantly in the cytoplasm, suggesting it may be utilizing non-genomic signalling. While exogenous oestrogen had no effect on proliferation or differentiation, we found some evidence for the endogenous production of oestrogen (intracrinology), as suggested by the expression of aromatase in these cells. Selective ERα blockade with methyl piperidinopyrazole (MPP) led to a significant reduction in both proliferation and differentiation, while ERβ blockade with R,R tetrahydrochrysene (THC) led to an increase in these parameters. This is in keeping with results from mouse knockout models suggesting that unopposed ERβ signalling leads to an inhibition of skeletal growth. Our results are further evidence for the importance of differential ER signalling in regulating chondrogenesis. Future studies examining in vivo effects of these agents are required to extrapolate these findings to a mammalian model.
    Keywords
    Endocrinology; Cell Physiology; Expanding Knowledge in the Biological Sciences; Expanding Knowledge in the Medical and Health Sciences

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