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dc.contributor.authorSimm, PJ
dc.contributor.authorRusso, VC
dc.contributor.authorWerther, GA
dc.identifier.citationSimm, P. J., Russo, V. C. & Werther, G. A. (2011). The effect of selective oestrogen receptor antagonists in an in vitro model of growth plate chondrogenesis. ENDOCRINE, 40 (1), pp.27-34.
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractWhile oestrogen is recognized to play a key role in regulating growth, particularly in relation to epiphyseal fusion, the mechanisms that mediate its effects are still unclear. We utilized an in vitro model of chondrogenesis, the RCJ3.1C5.18 cell line, to explore the effect of oestrogen on this process. We demonstrated the presence of oestrogen receptors (ER) α and β in these cells, with increased abundance of both receptor sub-types evident as the cells differentiated. ERα localized to the nucleus, suggesting it was signalling by genomic pathways, while ERβ was seen predominantly in the cytoplasm, suggesting it may be utilizing non-genomic signalling. While exogenous oestrogen had no effect on proliferation or differentiation, we found some evidence for the endogenous production of oestrogen (intracrinology), as suggested by the expression of aromatase in these cells. Selective ERα blockade with methyl piperidinopyrazole (MPP) led to a significant reduction in both proliferation and differentiation, while ERβ blockade with R,R tetrahydrochrysene (THC) led to an increase in these parameters. This is in keeping with results from mouse knockout models suggesting that unopposed ERβ signalling leads to an inhibition of skeletal growth. Our results are further evidence for the importance of differential ER signalling in regulating chondrogenesis. Future studies examining in vivo effects of these agents are required to extrapolate these findings to a mammalian model.
dc.publisherHUMANA PRESS INC
dc.subjectEndocrinology; Cell Physiology; Expanding Knowledge in the Biological Sciences; Expanding Knowledge in the Medical and Health Sciences
dc.titleThe effect of selective oestrogen receptor antagonists in an in vitro model of growth plate chondrogenesis
dc.typeJournal Article
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentPaediatrics Royal Children'S Hospital
melbourne.contributor.authorRusso, Vincent
melbourne.contributor.authorWerther, George
melbourne.contributor.authorSimm, Peter
melbourne.fieldofresearch320208 Endocrinology
melbourne.fieldofresearch320801 Cell physiology
melbourne.seocode280102 Expanding knowledge in the biological sciences
melbourne.accessrightsThis item is currently not available from this repository

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