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dc.contributor.authorSimm, PJ
dc.contributor.authorRusso, VC
dc.contributor.authorWerther, GA
dc.date.available2014-05-21T22:29:40Z
dc.date.available2011-04-07
dc.date.issued2011-08-01
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000293408900005&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=d4d813f4571fa7d6246bdc0dfeca3a1c
dc.identifier.citationSimm, P. J., Russo, V. C. & Werther, G. A. (2011). The effect of selective oestrogen receptor antagonists in an in vitro model of growth plate chondrogenesis. ENDOCRINE, 40 (1), pp.27-34. https://doi.org/10.1007/s12020-011-9473-2.
dc.identifier.issn1355-008X
dc.identifier.urihttp://hdl.handle.net/11343/29072
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractWhile oestrogen is recognized to play a key role in regulating growth, particularly in relation to epiphyseal fusion, the mechanisms that mediate its effects are still unclear. We utilized an in vitro model of chondrogenesis, the RCJ3.1C5.18 cell line, to explore the effect of oestrogen on this process. We demonstrated the presence of oestrogen receptors (ER) α and β in these cells, with increased abundance of both receptor sub-types evident as the cells differentiated. ERα localized to the nucleus, suggesting it was signalling by genomic pathways, while ERβ was seen predominantly in the cytoplasm, suggesting it may be utilizing non-genomic signalling. While exogenous oestrogen had no effect on proliferation or differentiation, we found some evidence for the endogenous production of oestrogen (intracrinology), as suggested by the expression of aromatase in these cells. Selective ERα blockade with methyl piperidinopyrazole (MPP) led to a significant reduction in both proliferation and differentiation, while ERβ blockade with R,R tetrahydrochrysene (THC) led to an increase in these parameters. This is in keeping with results from mouse knockout models suggesting that unopposed ERβ signalling leads to an inhibition of skeletal growth. Our results are further evidence for the importance of differential ER signalling in regulating chondrogenesis. Future studies examining in vivo effects of these agents are required to extrapolate these findings to a mammalian model.
dc.languageEnglish
dc.publisherHUMANA PRESS INC
dc.subjectEndocrinology; Cell Physiology; Expanding Knowledge in the Biological Sciences; Expanding Knowledge in the Medical and Health Sciences
dc.titleThe effect of selective oestrogen receptor antagonists in an in vitro model of growth plate chondrogenesis
dc.typeJournal Article
dc.identifier.doi10.1007/s12020-011-9473-2
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentPaediatrics Royal Children'S Hospital
melbourne.source.titleENDOCRINE
melbourne.source.volume40
melbourne.source.issue1
melbourne.source.pages27-34
dc.research.codefor110306
dc.research.codefor111601
dc.research.codeseo2008970106
dc.research.codeseo2008970111
melbourne.publicationid168201
melbourne.elementsid337769
melbourne.contributor.authorRusso, Vincent
melbourne.contributor.authorWerther, George
melbourne.contributor.authorSimm, Peter
dc.identifier.eissn1559-0100
pubs.acceptance.date2011-04-07
melbourne.accessrightsThis item is currently not available from this repository


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