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dc.contributor.authorNg, Ashley P.en_US
dc.contributor.authorKAUPPI, MARIAen_US
dc.contributor.authorMETCALF, DONALDen_US
dc.contributor.authorDi Rago, Ladinaen_US
dc.contributor.authorHyland, Craig D.en_US
dc.contributor.authorALEXANDER, WARRENen_US
dc.date.accessioned2014-05-21T22:30:21Z
dc.date.available2014-05-21T22:30:21Z
dc.date.issued2012en_US
dc.identifier.citationNg, A. P., Kauppi, M., Metcalf, D., Di Rago, L., Hyland, C. D. & Alexander, W. S. (2012). Characterization of TPO-responsive progenitor cells in adult mouse bone marrow with in vivo megakaryocyte and erythroid potential. Proceedings of the National Academy of Sciences of the United States of America. doi: 10.1073/pnas.1121385109.en_US
dc.identifier.urihttp://hdl.handle.net/11343/29079
dc.description© 2012 Ashley P. Ng, Maria Kauppi, Donald Metcalf, Ladina Di Rago, Craig D. Hyland & Warren S. Alexanderen_US
dc.descriptionPublished as “Characterization of thrombopoietin (TPO)-responsive progenitor cells in adult mouse bone marrow with in vivo megakaryocyte and erythroid potential” in Proceedings of the National Academy of Sciences (PNAS) of the United States of America. The definitive version is available at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3289343/en_US
dc.description.abstractHematopoietic progenitor cells are the progeny of hematopoietic stem cells that coordinate production of precise numbers of mature blood cells of diverse functional lineages. Identification of cell surface antigen expression associated with hematopoietic lineage restriction has allowed prospective isolation of progenitor cells with defined hematopoietic potential. To further clarify the cellular origins of megakaryocyte commitment, we have assessed the in vitro and in vivo megakaryocyte and platelet potential of defined progenitor populations in the adult mouse bone marrow. We show that megakaryocytes arise from CD150+ bipotential progenitors that display both platelet- and erythrocyte-producing potential in vivo, and that can develop from the Flt3- fraction of the pre-granulocyte-macrophage (PreGM) population. We define a bi-potential erythroid-megakaryocyte progenitor population (BEMP), the CD150+CD9loEndoglinlo fraction of Lin-cKit+IL7Rα-FcγRII/IIIloSca1- cells, which contains the bulk of the megakaryocyte colony-forming capacity (CFC) of the bone marrow, including bipotential megakaryocyte-erythroid CFC, and can efficiently generate both erythrocytes and platelets in vivo. This fraction is distinct from the CD150+CD9hiEndoglinlo fraction, which contains bipotential precursors with characteristics of increased megakaryocytic maturation, and the CD150+CD9loEndoglinhi fraction, which contains erythroid lineage-committed cells. Finally, we demonstrate that BEMP and CD150+CD9hiEndoglinlo cells are TPO-responsive and that the latter population specifically expands in the recovery from thrombocytopenia induced by anti-platelet serum.en_US
dc.languageengen_US
dc.publisherNational Academy of Sciences of the United States of Americaen_US
dc.subjectthrombopoietinen_US
dc.subjectmegakaryocyteen_US
dc.subjectprogenitoren_US
dc.titleCharacterization of TPO-responsive progenitor cells in adult mouse bone marrow with in vivo megakaryocyte and erythroid potentialen_US
dc.typeJournal Articleen_US
dc.identifier.doi10.1073/pnas.1121385109
melbourne.peerreviewPeer Revieweden_US
melbourne.affiliationThe University of Melbourneen_US
melbourne.affiliation.departmentThe Walter and Eliza Hall Institute of Medical Researchen_US
melbourne.affiliation.departmentDepartment of Medical Biologyen_US
melbourne.publication.statusIn Pressen_US
melbourne.source.titleProceedings of the National Academy of Sciences of the United States of Americaen_US
melbourne.elementsidNA
melbourne.contributor.authorNg, Ashley
melbourne.contributor.authorKauppi, Maria
melbourne.contributor.authorMETCALF, DONALD
melbourne.contributor.authorAlexander, Warren
melbourne.accessrightsOpen Access


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