Show simple item record

dc.contributor.authorJames, SA
dc.contributor.authorMyers, DE
dc.contributor.authorde Jonge, MD
dc.contributor.authorVogt, S
dc.contributor.authorRyan, CG
dc.contributor.authorSexton, BA
dc.contributor.authorHoobin, P
dc.contributor.authorPaterson, D
dc.contributor.authorHoward, DL
dc.contributor.authorMayo, SC
dc.contributor.authorAltissimo, M
dc.contributor.authorMoorhead, GF
dc.contributor.authorWilkins, SW
dc.identifier.citationJames, S. A., Myers, D. E., de Jonge, M. D., Vogt, S., Ryan, C. G., Sexton, B. A., Hoobin, P., Paterson, D., Howard, D. L., Mayo, S. C., Altissimo, M., Moorhead, G. F. & Wilkins, S. W. (2011). Quantitative comparison of preparation methodologies for x-ray fluorescence microscopy of brain tissue. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 401 (3), pp.853-864.
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractX-ray fluorescence microscopy (XFM) facilitates high-sensitivity quantitative imaging of trace metals at high spatial resolution over large sample areas and can be applied to a diverse range of biological samples. Accurate determination of elemental content from recorded spectra requires proper calibration of the XFM instrument under the relevant operating conditions. Here, we describe the manufacture, characterization, and utilization of multi-element thin-film reference foils for use in calibration of XFM measurements of biological and other specimens. We have used these internal standards to assess the two-dimensional distribution of trace metals in a thin tissue section of a rat hippocampus. The data used in this study was acquired at the XFM beamline of the Australian Synchrotron using a new 384-element array detector (Maia) and at beamline 2-ID-E at the Advanced Photon Source. Post-processing of samples by different fixation techniques was investigated, with the conclusion that differences in solvent type and sample handling can significantly alter elemental content. The present study highlights the quantitative capability, high statistical power, and versatility of the XFM technique for mapping trace metals in biological samples, e.g., brain tissue samples in order to help understand neurological processes, especially when implemented in conjunction with a high-performance detector such as Maia.
dc.subjectClinical Chemistry (diagnostics); Nervous System and Disorders
dc.titleQuantitative comparison of preparation methodologies for x-ray fluorescence microscopy of brain tissue
dc.typeJournal Article
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentSurgery - St Vincent'S Hospital
melbourne.contributor.authorMyers, Damian
melbourne.contributor.authorRyan, Christopher
melbourne.contributor.authorMOORHEAD, GARETH
melbourne.contributor.authorJames, Simon
melbourne.fieldofresearch320202 Clinical chemistry (incl. diagnostics)
melbourne.seocode200199 Clinical health not elsewhere classified
melbourne.accessrightsThis item is currently not available from this repository

Files in this item


There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record