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    Subcompartmentalisation of Proteins in the Rhoptries Correlates with Ordered Events of Erythrocyte Invasion by the Blood Stage Malaria Parasite

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    Subcompartmentalisation of proteins in the rhoptries correlates with ordered events of erythrocyte invasion by the blood stage malaria parasite (5.885Mb)

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    Author
    Zuccala, ES; Gout, AM; Dekiwadia, C; Marapana, DS; Angrisano, F; Turnbull, L; Riglar, DT; Rogers, KL; Whitchurch, CB; Ralph, SA; ...
    Date
    2012-09-25
    Source Title
    PLOS ONE
    Publisher
    PUBLIC LIBRARY SCIENCE
    University of Melbourne Author/s
    DEKIWADIA, CHAITALI; Speed, Terence; BAUM, JACOB; Ralph, Stuart; Rogers, Kelly; RIGLAR, DAVID; GOUT, ALEXANDER MATHEW; Zuccala, Elizabeth; Angrisano, Fiona
    Affiliation
    Infection and Immunity, Walter and Eliza Hall Institute of Medical Research
    Bioinformatics Divisions, Walter and Eliza Hall Institute of Medical Research
    Imaging Facility, Walter and Eliza Hall Institute of Medical Research
    Department of Medical Biology
    Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute
    Metadata
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    Document Type
    Journal Article
    Citations
    Zuccala, E. S., Gout, A. M., Dekiwadia, C., Marapana, D. S., Angrisano, F., Turnbull, L., Riglar, D. T., Rogers, K. L., Whitchurch, C. B., Ralph, S. A., Speed, T. P. & Baum, J. (2012). Subcompartmentalisation of Proteins in the Rhoptries Correlates with Ordered Events of Erythrocyte Invasion by the Blood Stage Malaria Parasite. PLOS ONE, 7 (9), https://doi.org/10.1371/journal.pone.0046160.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/32738
    DOI
    10.1371/journal.pone.0046160
    Open Access at PMC
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3458004
    Description

    © 2012 Zuccala et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/3.0

     

    The research outputs in this collection have been funded in whole or in part by the National Health and Medical Research Council (NHMRC)

     
    Abstract
    Host cell infection by apicomplexan parasites plays an essential role in lifecycle progression for these obligate intracellular pathogens. For most species, including the etiological agents of malaria and toxoplasmosis, infection requires active host-cell invasion dependent on formation of a tight junction - the organising interface between parasite and host cell during entry. Formation of this structure is not, however, shared across all Apicomplexa or indeed all parasite lifecycle stages. Here, using an in silico integrative genomic search and endogenous gene-tagging strategy, we sought to characterise proteins that function specifically during junction-dependent invasion, a class of proteins we term invasins to distinguish them from adhesins that function in species specific host-cell recognition. High-definition imaging of tagged Plasmodium falciparum invasins localised proteins to multiple cellular compartments of the blood stage merozoite. This includes several that localise to distinct subcompartments within the rhoptries. While originating from the same organelle, however, each has very different dynamics during invasion. Apical Sushi Protein and Rhoptry Neck protein 2 release early, following the junction, whilst a novel rhoptry protein PFF0645c releases only after invasion is complete. This supports the idea that organisation of proteins within a secretory organelle determines the order and destination of protein secretion and provides a localisation-based classification strategy for predicting invasin function during apicomplexan parasite invasion.
    Keywords
    cell infection; intracellular pathogens; malaria parasite; proteins

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