Show simple item record

dc.contributor.authorZuccala, ES
dc.contributor.authorGout, AM
dc.contributor.authorDekiwadia, C
dc.contributor.authorMarapana, DS
dc.contributor.authorAngrisano, F
dc.contributor.authorTurnbull, L
dc.contributor.authorRiglar, DT
dc.contributor.authorRogers, KL
dc.contributor.authorWhitchurch, CB
dc.contributor.authorRalph, SA
dc.contributor.authorSpeed, TP
dc.contributor.authorBaum, J
dc.date.available2014-05-22T06:55:20Z
dc.date.available2012-08-27
dc.date.available2012-08-27
dc.date.available2012-08-27
dc.date.issued2012-09-25
dc.identifierpii: PONE-D-12-18651
dc.identifier.citationZuccala, E. S., Gout, A. M., Dekiwadia, C., Marapana, D. S., Angrisano, F., Turnbull, L., Riglar, D. T., Rogers, K. L., Whitchurch, C. B., Ralph, S. A., Speed, T. P. & Baum, J. (2012). Subcompartmentalisation of Proteins in the Rhoptries Correlates with Ordered Events of Erythrocyte Invasion by the Blood Stage Malaria Parasite. PLOS ONE, 7 (9), https://doi.org/10.1371/journal.pone.0046160.
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/11343/32738
dc.description© 2012 Zuccala et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. http://creativecommons.org/licenses/by/3.0
dc.descriptionThe research outputs in this collection have been funded in whole or in part by the National Health and Medical Research Council (NHMRC)
dc.description.abstractHost cell infection by apicomplexan parasites plays an essential role in lifecycle progression for these obligate intracellular pathogens. For most species, including the etiological agents of malaria and toxoplasmosis, infection requires active host-cell invasion dependent on formation of a tight junction - the organising interface between parasite and host cell during entry. Formation of this structure is not, however, shared across all Apicomplexa or indeed all parasite lifecycle stages. Here, using an in silico integrative genomic search and endogenous gene-tagging strategy, we sought to characterise proteins that function specifically during junction-dependent invasion, a class of proteins we term invasins to distinguish them from adhesins that function in species specific host-cell recognition. High-definition imaging of tagged Plasmodium falciparum invasins localised proteins to multiple cellular compartments of the blood stage merozoite. This includes several that localise to distinct subcompartments within the rhoptries. While originating from the same organelle, however, each has very different dynamics during invasion. Apical Sushi Protein and Rhoptry Neck protein 2 release early, following the junction, whilst a novel rhoptry protein PFF0645c releases only after invasion is complete. This supports the idea that organisation of proteins within a secretory organelle determines the order and destination of protein secretion and provides a localisation-based classification strategy for predicting invasin function during apicomplexan parasite invasion.
dc.languageEnglish
dc.publisherPUBLIC LIBRARY SCIENCE
dc.subjectcell infection
dc.subjectintracellular pathogens
dc.subjectmalaria parasite
dc.subjectproteins
dc.titleSubcompartmentalisation of Proteins in the Rhoptries Correlates with Ordered Events of Erythrocyte Invasion by the Blood Stage Malaria Parasite
dc.typeJournal Article
dc.identifier.doi10.1371/journal.pone.0046160
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentInfection and Immunity, Walter and Eliza Hall Institute of Medical Research
melbourne.affiliation.departmentBioinformatics Divisions, Walter and Eliza Hall Institute of Medical Research
melbourne.affiliation.departmentImaging Facility, Walter and Eliza Hall Institute of Medical Research
melbourne.affiliation.departmentDepartment of Medical Biology
melbourne.affiliation.departmentDepartment of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute
melbourne.source.titlePLOS ONE
melbourne.source.volume7
melbourne.source.issue9
dc.rights.licenseCC BY
melbourne.publicationid187281
dc.description.doi10.1371/journal.pone.0046160
melbourne.elementsid410565
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3458004
melbourne.contributor.authorDEKIWADIA, CHAITALI
melbourne.contributor.authorSpeed, Terence
melbourne.contributor.authorBAUM, JACOB
melbourne.contributor.authorRalph, Stuart
melbourne.contributor.authorRogers, Kelly
melbourne.contributor.authorRIGLAR, DAVID
melbourne.contributor.authorGOUT, ALEXANDER MATHEW
melbourne.contributor.authorZuccala, Elizabeth
melbourne.contributor.authorAngrisano, Fiona
dc.identifier.eissn1932-6203
melbourne.accessrightsOpen Access


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record