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dc.contributor.authorZhan, Y
dc.contributor.authorVega-Ramos, J
dc.contributor.authorCarrington, EM
dc.contributor.authorVilladangos, JA
dc.contributor.authorLew, AM
dc.contributor.authorXu, Y
dc.date.available2014-05-22T06:56:46Z
dc.date.available2012-07-12
dc.date.available2012-07-12
dc.date.available2012-07-12
dc.date.available2012-07-12
dc.date.available2012-07-12
dc.date.available2012-07-12
dc.date.available2012-07-12
dc.date.available2012-07-12
dc.date.available2012-07-12
dc.date.issued2012-11-01
dc.identifierhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000310544000009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=d4d813f4571fa7d6246bdc0dfeca3a1c
dc.identifier.citationZhan, Y., Vega-Ramos, J., Carrington, E. M., Villadangos, J. A., Lew, A. M. & Xu, Y. (2012). The inflammatory cytokine, GM-CSF, alters the developmental outcome of murine dendritic cells. EUROPEAN JOURNAL OF IMMUNOLOGY, 42 (11), pp.2889-2900. https://doi.org/10.1002/eji.201242477.
dc.identifier.issn0014-2980
dc.identifier.urihttp://hdl.handle.net/11343/32746
dc.description© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
dc.descriptionThe research outputs in this collection have been funded in whole or in part by the National Health and Medical Research Council (NHMRC).
dc.description.abstractFms-like tyrosine kinase 3 ligand (Flt3L) is a major cytokine that drives development of dendritic cells (DCs) under steady state, whereas GM-CSF becomes a prominent influence on differentiation during inflammation. The influence GM-CSF exerts on Flt3L-induced DC development has not been thoroughly examined. Here, we report that GM-CSF alters Flt3L-induced DC development. When BM cells were cultured with both Flt3L and GM-CSF, few CD8⁺ equivalent DCs or plasmacytoid DCs developed compared to cultures supplemented with Flt3L alone. The disappearance of these two cell subsets in GM-CSF + Flt3L culture was not a result of simple inhibition of their development, but a diversion of the original differentiation trajectory to form a new cell population. As a consequence, both DC progeny and their functions were altered. The effect of GM-CSF on DC subset development was confirmed in vivo. First, the CD8⁺ DC numbers were increased under GM-CSF deficiency (when either GM-CSF or its receptor was ablated). Second, this population was decreased under GM-CSF hyperexpression (by transgenesis or by Listeria infection). Our finding that GM-CSF dominantly changes the regulation of DC development in vitro and in vivo has important implications for inflammatory diseases or GM-CSF therapy.
dc.languageEnglish
dc.publisherWILEY
dc.subjectGM-CSF
dc.subjectFlt3L
dc.subjectdendritic cells and differentiation
dc.titleThe inflammatory cytokine, GM-CSF, alters the developmental outcome of murine dendritic cells
dc.typeJournal Article
dc.identifier.doi10.1002/eji.201242477
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentThe Walter and Eliza Hall Institute of Medical Research
melbourne.affiliation.departmentDepartment of Medical Biology
melbourne.affiliation.departmentDepartment of Biochemistry and Molecular Biology
melbourne.source.titleEUROPEAN JOURNAL OF IMMUNOLOGY
melbourne.source.volume42
melbourne.source.issue11
melbourne.source.pages2889-2900
melbourne.identifier.nhmrc1006428
melbourne.publicationid187955
dc.description.doi10.1002/eji.201242477
melbourne.elementsid357613
melbourne.contributor.authorXU, YUEKANG
melbourne.contributor.authorCarrington, Emma
melbourne.contributor.authorVilladangos, Jose
melbourne.contributor.authorLew, Andrew
melbourne.contributor.authorZhan, Yifan
melbourne.contributor.authorVEGA RAMOS, JAVIER
dc.identifier.eissn1521-4141
melbourne.identifier.fundernameidNHMRC, 1006428
pubs.acceptance.date2012-07-12
melbourne.accessrightsThis item is currently not available from this repository


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