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    Electroretinography in streptozotocin diabetic rats following acute intraocular pressure elevation

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    Author
    Kohzaki, K; Vingrys, AJ; Armitage, JA; Bui, BV
    Date
    2013-02-01
    Source Title
    GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY
    Publisher
    SPRINGER
    University of Melbourne Author/s
    Vingrys, Algis; Bui, Bang; Cole, Barry
    Affiliation
    Optometry And Vision Sciences
    Metadata
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    Document Type
    Journal Article
    Citations
    Kohzaki, K., Vingrys, A. J., Armitage, J. A. & Bui, B. V. (2013). Electroretinography in streptozotocin diabetic rats following acute intraocular pressure elevation. GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 251 (2), pp.529-535. https://doi.org/10.1007/s00417-012-2212-4.
    Access Status
    Open Access
    URI
    http://hdl.handle.net/11343/32894
    DOI
    10.1007/s00417-012-2212-4
    NHMRC Grant code
    NHMRC/400127
    NHMRC/350224
    NHMRC/350224
    Description

    C1 - Journal Articles Refereed

    Abstract
    BACKGROUND: We consider whether pre-existing streptozotocin induced hyperglycemia in rats affects the ability of the eye to cope with a single episode of acute intraocular pressure (IOP) elevation. METHODS: Electroretinogram (ERG) responses were measured (-6.08 to 1.92 log cd.s.m(-2)) in anaesthetized (60:5 mg/kg ketamine:xylazine) dark-adapted (>12 h) adult Sprague-Dawley rats 1 week after a single acute IOP elevation to 70 mmHg for 60 min. This was undertaken in rats treated 11 weeks earlier with streptozotocin (STZ, n = 12, 50 mg/kg at 6 weeks of age) or citrate buffer (n = 12). ERG responses were analyzed to derive an index of photoreceptor (a-wave), ON-bipolar (b-wave), amacrine (oscillatory potentials) and inner retinal (positive scotopic threshold response, pSTR) function. RESULTS: One week following acute IOP elevation there was a significant reduction of the ganglion cell pSTR (-35 ± 11 %, P = 0.0161) in STZ-injected animals. In contrast the pSTR in citrate-injected animals was not significant changed (+16 ± 14 %). The negative component of the STR was unaffected by IOP elevation in either citrate or STZ-treated groups. Photoreceptoral (a-wave, citrate-control +4 ± 3 %, STZ +4 ± 5 %) and ON-bipolar cell (b-wave, control +4 ± 3 %, STZ +4 ± 5 %) mediated responses were not significantly affected by IOP elevation in either citrate- or STZ-injected rats. Finally, oscillatory potentials (citrate-control +8 ± 23 %, STZ +1 ± 17 %) were not reduced 1 week after IOP challenge. CONCLUSIONS: The ganglion cell dominated pSTR was reduced following a single episode of IOP elevation in STZ diabetic, but not control rats. These data indicate that hyperglycemia renders the inner retina more susceptible to IOP elevation.
    Keywords
    Optometry and Ophthalmology not elsewhere classified; Hearing; Vision; Speech and Their Disorders

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