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dc.contributor.authorKohzaki, K
dc.contributor.authorVingrys, AJ
dc.contributor.authorArmitage, JA
dc.contributor.authorBui, BV
dc.date.available2014-05-22T07:23:32Z
dc.date.issued2013-02-01
dc.identifier.citationKohzaki, K., Vingrys, A. J., Armitage, J. A. & Bui, B. V. (2013). Electroretinography in streptozotocin diabetic rats following acute intraocular pressure elevation. GRAEFES ARCHIVE FOR CLINICAL AND EXPERIMENTAL OPHTHALMOLOGY, 251 (2), pp.529-535. https://doi.org/10.1007/s00417-012-2212-4.
dc.identifier.issn0721-832X
dc.identifier.urihttp://hdl.handle.net/11343/32894
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractBACKGROUND: We consider whether pre-existing streptozotocin induced hyperglycemia in rats affects the ability of the eye to cope with a single episode of acute intraocular pressure (IOP) elevation. METHODS: Electroretinogram (ERG) responses were measured (-6.08 to 1.92 log cd.s.m(-2)) in anaesthetized (60:5 mg/kg ketamine:xylazine) dark-adapted (>12 h) adult Sprague-Dawley rats 1 week after a single acute IOP elevation to 70 mmHg for 60 min. This was undertaken in rats treated 11 weeks earlier with streptozotocin (STZ, n = 12, 50 mg/kg at 6 weeks of age) or citrate buffer (n = 12). ERG responses were analyzed to derive an index of photoreceptor (a-wave), ON-bipolar (b-wave), amacrine (oscillatory potentials) and inner retinal (positive scotopic threshold response, pSTR) function. RESULTS: One week following acute IOP elevation there was a significant reduction of the ganglion cell pSTR (-35 ± 11 %, P = 0.0161) in STZ-injected animals. In contrast the pSTR in citrate-injected animals was not significant changed (+16 ± 14 %). The negative component of the STR was unaffected by IOP elevation in either citrate or STZ-treated groups. Photoreceptoral (a-wave, citrate-control +4 ± 3 %, STZ +4 ± 5 %) and ON-bipolar cell (b-wave, control +4 ± 3 %, STZ +4 ± 5 %) mediated responses were not significantly affected by IOP elevation in either citrate- or STZ-injected rats. Finally, oscillatory potentials (citrate-control +8 ± 23 %, STZ +1 ± 17 %) were not reduced 1 week after IOP challenge. CONCLUSIONS: The ganglion cell dominated pSTR was reduced following a single episode of IOP elevation in STZ diabetic, but not control rats. These data indicate that hyperglycemia renders the inner retina more susceptible to IOP elevation.
dc.languageEnglish
dc.publisherSPRINGER
dc.subjectOptometry and Ophthalmology not elsewhere classified; Hearing
dc.subjectVision
dc.subjectSpeech and Their Disorders
dc.titleElectroretinography in streptozotocin diabetic rats following acute intraocular pressure elevation
dc.typeJournal Article
dc.identifier.doi10.1007/s00417-012-2212-4
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentOptometry And Vision Sciences
melbourne.source.titleGraefes Archive for Clinical and Experimental Ophthalmology
melbourne.source.volume251
melbourne.source.issue2
melbourne.source.pages529-535
melbourne.identifier.nhmrc400127
melbourne.identifier.nhmrc350224
melbourne.identifier.nhmrc350224
melbourne.publicationid185987
melbourne.elementsid498964
melbourne.contributor.authorVingrys, Algis
melbourne.contributor.authorBui, Bang
melbourne.contributor.authorCole, Barry
dc.identifier.eissn1435-702X
melbourne.identifier.fundernameidNHMRC, 400127
melbourne.identifier.fundernameidNHMRC, 350224
melbourne.identifier.fundernameidNHMRC, 350224
melbourne.fieldofresearch321299 Ophthalmology and optometry not elsewhere classified
melbourne.seocode200199 Clinical health not elsewhere classified
melbourne.accessrightsOpen Access


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