Susceptibility of Streptozotocin-Induced Diabetic Rat Retinal Function and Ocular Blood Flow to Acute Intraocular Pressure Challenge
AuthorWong, VHY; Vingrys, AJ; Jobling, AI; Bui, BV
Source TitleInvestigative Ophthalmology and Visual Science
PublisherASSOC RESEARCH VISION OPHTHALMOLOGY INC
University of Melbourne Author/sBui, Bang; Jobling, Andrew; Vingrys, Algis; WONG, VICKIE; Wong, Vickie; Cole, Barry
AffiliationAnatomy and Neuroscience
Optometry and Vision Sciences
Document TypeJournal Article
CitationsWong, V. H. Y., Vingrys, A. J., Jobling, A. I. & Bui, B. V. (2013). Susceptibility of Streptozotocin-Induced Diabetic Rat Retinal Function and Ocular Blood Flow to Acute Intraocular Pressure Challenge. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 54 (3), pp.2133-2141. https://doi.org/10.1167/iovs.13-11595.
Access StatusThis item is currently not available from this repository
NHMRC Grant codeNHMRC/566570
C1 - Journal Articles Refereed
PURPOSE: To consider the hypothesis that streptozotocin (STZ)-induced hyperglycemia renders rat retinal function and ocular blood flow more susceptible to acute IOP challenge. METHODS: Retinal function (electroretinogram [ERG]) was measured during acute IOP challenge (10100 mm Hg, increments of 5 mm Hg, 3 minutes per step, vitreal cannulation) in adult Long-Evans rats (6 weeks old; citrate: n = 6, STZ: n = 10) 4 weeks after citrate buffer or STZ (65 mg/kg, blood glucose >15 mM) injection. At each IOP, dim and bright flash (-4.56, -1.72 log cd x s x m(-2)) ERG responses were recorded to measure inner retinal and ON-bipolar cell function, respectively. Ocular blood flow (laser Doppler flowmetry; citrate: n = 6, STZ: n = 10) was also measured during acute IOP challenge. Retinas were isolated for quantitative PCR analysis of nitric oxide synthase mRNA expression (endothelial, eNos; inducible, iNos; neuronal, nNos). RESULTS: STZ-induced diabetes increased the susceptibility of inner retinal (IOP at 50% response, 60.1, CI: 57.0-62.0 mm Hg versus citrate: 67.5, CI: 62.1-72.4 mm Hg) and ON-bipolar cell function (STZ: 60.3, CI: 58.0-62.8 mm Hg versus citrate: 65.1, CI: 61.9-68.6 mm Hg) and ocular blood flow (43.9, CI: 40.8-46.8 versus citrate: 53.4, CI: 50.7-56.1 mm Hg) to IOP challenge. Citrate eyes showed elevated eNos mRNA (+49.7%) after IOP stress, an effect not found in STZ-diabetic eyes (-5.7%, P < 0.03). No difference was observed for iNos or nNos (P > 0.05) following IOP elevation. CONCLUSIONS: STZ-induced diabetes increased functional susceptibility during acute IOP challenge. This functional vulnerability is associated with a reduced capacity for diabetic eyes to upregulate eNos expression and to autoregulate blood flow in response to stress.
KeywordsOptometry and Ophthalmology not elsewhere classified; Diabetes; Hearing; Vision; Speech and Their Disorders
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