Calcium imaging of neuronal activity in the most rostral parafacial respiratory group of the newborn rat
AuthorOnimaru, H; Dutschmann, M
Source TitleJOURNAL OF PHYSIOLOGICAL SCIENCES
PublisherSPRINGER JAPAN KK
University of Melbourne Author/sDutschmann, Mathias
AffiliationAnatomy And Neuroscience
Document TypeJournal Article
CitationsOnimaru, H. & Dutschmann, M. (2012). Calcium imaging of neuronal activity in the most rostral parafacial respiratory group of the newborn rat. JOURNAL OF PHYSIOLOGICAL SCIENCES, 62 (1), pp.71-77. https://doi.org/10.1007/s12576-011-0179-2.
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C1 - Journal Articles Refereed
The parafacial respiratory group (pFRG) is thought to be involved in respiratory rhythm generation in neonates. This subgroup expresses the transcription factor, Phox2b, and contains intrinsically CO(2) sensitive neurons. Calcium imaging has been widely used for analysis of neuronal activity at the cellular and network level. In the present study, we applied calcium imaging to analyze neuronal activity of the most-rostral pFRG in an in vitro brainstem-spinal cord preparation from neonatal rats. We detected strong pre-inspiratory neuron activity in the most rostral pFRG, suggesting that significant numbers of pre-inspiratory neurons are localized in the ventrolateral medulla near the rostral end of the medulla. We show that usage of calcium imaging would be very useful for analysis of neuronal activity over different time scales, and discuss the advantages and disadvantages of this method.
KeywordsNeurosciences not elsewhere classified; Clinical Health (Organs; Diseases and Abnormal Conditions) not elsewhere classified
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