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dc.contributor.authorStatham, AL
dc.contributor.authorRobinson, MD
dc.contributor.authorSong, JZ
dc.contributor.authorCoolen, MW
dc.contributor.authorStirzaker, C
dc.contributor.authorClark, SJ
dc.date.available2014-05-22T08:30:54Z
dc.date.issued2012-06-01
dc.identifierpii: gr.132076.111
dc.identifier.citationStatham, A. L., Robinson, M. D., Song, J. Z., Coolen, M. W., Stirzaker, C. & Clark, S. J. (2012). Bisulfite sequencing of chromatin immunoprecipitated DNA (BisChIP-seq) directly informs methylation status of histone-modified DNA. GENOME RESEARCH, 22 (6), pp.1120-1127. https://doi.org/10.1101/gr.132076.111.
dc.identifier.issn1088-9051
dc.identifier.urihttp://hdl.handle.net/11343/33261
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractThe complex relationship between DNA methylation, chromatin modification, and underlying DNA sequence is often difficult to unravel with existing technologies. Here, we describe a novel technique based on high-throughput sequencing of bisulfite-treated chromatin immunoprecipitated DNA (BisChIP-seq), which can directly interrogate genetic and epigenetic processes that occur in normal and diseased cells. Unlike most previous reports based on correlative techniques, we found using direct bisulfite sequencing of Polycomb H3K27me3-enriched DNA from normal and prostate cancer cells that DNA methylation and H3K27me3-marked histones are not always mutually exclusive, but can co-occur in a genomic region-dependent manner. Notably, in cancer, the co-dependency of marks is largely redistributed with an increase of the dual repressive marks at CpG islands and transcription start sites of silent genes. In contrast, there is a loss of DNA methylation in intergenic H3K27me3-marked regions. Allele-specific methylation status derived from the BisChIP-seq data clearly showed that both methylated and unmethylated alleles can simultaneously be associated with H3K27me3 histones, highlighting that DNA methylation status in these regions is not dependent on Polycomb chromatin status. BisChIP-seq is a novel approach that can be widely applied to directly interrogate the genomic relationship between allele-specific DNA methylation, histone modification, or other important epigenetic regulators.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherCOLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
dc.subjectEpigenetics (incl. Genome Methylation and Epigenomics); Cancer Genetics; Cancer Cell Biology; Cancer and Related Disorders
dc.titleBisulfite sequencing of chromatin immunoprecipitated DNA (BisChIP-seq) directly informs methylation status of histone-modified DNA
dc.typeJournal Article
dc.identifier.doi10.1101/gr.132076.111
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentMedical Biology (W.E.H.I.)
melbourne.source.titleGENOME RESEARCH
melbourne.source.volume22
melbourne.source.issue6
melbourne.source.pages1120-1127
dc.research.codefor060404
dc.research.codefor111203
dc.research.codefor111201
dc.research.codeseo2008920102
dc.rights.licenseCC BY-NC
melbourne.publicationid197094
melbourne.elementsid441970
melbourne.openaccess.pmchttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3371705
melbourne.contributor.authorROBINSON, MARK DARRELL
dc.identifier.eissn1549-5469
melbourne.accessrightsAccess this item via the Open Access location


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