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dc.contributor.authorJanardhana, V
dc.contributor.authorTachedjian, M
dc.contributor.authorCrameri, G
dc.contributor.authorCowled, C
dc.contributor.authorWang, L-F
dc.contributor.authorBaker, ML
dc.date.available2014-05-22T08:31:05Z
dc.date.available2011-11-01
dc.date.available2011-11-01
dc.date.available2011-11-01
dc.date.issued2012-03-01
dc.identifierpii: S0145-305X(11)00300-4
dc.identifier.citationJanardhana, V., Tachedjian, M., Crameri, G., Cowled, C., Wang, L. -F. & Baker, M. L. (2012). Cloning, expression and antiviral activity of IFN gamma from the Australian fruit bat, Pteropus alecto. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 36 (3), pp.610-618. https://doi.org/10.1016/j.dci.2011.11.001.
dc.identifier.issn0145-305X
dc.identifier.urihttp://hdl.handle.net/11343/33262
dc.descriptionC1 - Journal Articles Refereed
dc.description.abstractBats are natural reservoir hosts to a variety of viruses, many of which cause morbidity and mortality in other mammals. Currently there is a paucity of information regarding the nature of the immune response to viral infections in bats, partly due to a lack of appropriate bat specific reagents. IFNγ plays a key role in controlling viral replication and coordinating a response for long term control of viral infection. Here we describe the cloning and expression of IFNγ from the Australian flying fox, Pteropus alecto and the generation of mouse monoclonal and chicken egg yolk antibodies specific to bat IFNγ. Our results demonstrate that P. alecto IFNγ is conserved with IFNγ from other species and is induced in bat splenocytes following stimulation with T cell mitogens. P. alecto IFNγ has antiviral activity on Semliki forest virus in cell lines from P. alecto and the microbat, Tadarida brasiliensis. Additionally recombinant bat IFNγ was able to mitigate Hendra virus infection in P. alecto cells. These results provide the first evidence for an antiviral role for bat IFNγin vitro in addition to the application of important immunological reagents for further studies of bat antiviral immunity.
dc.formatapplication/pdf
dc.languageEnglish
dc.publisherELSEVIER SCI LTD
dc.subjectMedical Virology; Infectious Diseases
dc.titleCloning, expression and antiviral activity of IFN gamma from the Australian fruit bat, Pteropus alecto
dc.typeJournal Article
dc.identifier.doi10.1016/j.dci.2011.11.001
melbourne.peerreviewPeer Reviewed
melbourne.affiliationThe University of Melbourne
melbourne.affiliation.departmentMicrobiology And Immunology
melbourne.source.titleDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
melbourne.source.volume36
melbourne.source.issue3
melbourne.source.pages610-618
dc.research.codefor110804
dc.research.codeseo2008920109
melbourne.publicationid196178
melbourne.elementsid407830
melbourne.contributor.authorWANG, LINFA
dc.identifier.eissn1879-0089
melbourne.conference.locationUnited States
pubs.acceptance.date2011-11-01
melbourne.accessrightsThis item is currently not available from this repository


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