Development of rapid strain typing of aedes mosquito by random amplified polymorphic DNA (RAPD)
AuthorKoh, Mrs Siok Im; Yap, Dr Eric; Chia, Mr Yeong Chit; Ling, Mr Maurice HT; Wong, Mr Edwin CK
AffiliationScience: Department of Zoology
CitationsKoh, Mrs Siok Im and Yap, Dr Eric and Chia, Mr Yeong Chit and Ling, Mr Maurice HT and Wong, Mr Edwin CK (1999) Development of rapid strain typing of aedes mosquito by random amplified polymorphic DNA (RAPD).
Access StatusOpen Access
In this study, we were able to identify individual mosquito larva of Aedes aegypti in terms of their discrete ability to function as vector for Dengue virus using RAPD fingerprinting of genomic DNA. Four different methodologies of genomic DNA isoltation were evaulated based on economy and efficiency and 24 hour SDS lysis satisfied both criterias. Nine 10-base primers were evaluated and only five of them, A2, B1, C13, AA1 and AA3, were found potentially suitable for RAPD fingerprinting. All fingerprinting profiles were analyzed by a in-house designed artificial intelligence software but only those of A1 and B1 were analyzed using Similarity Index and Percent Matching. Out of these three methods, only the results from the software were highly promising. The best result was obtained from profiles by primer C13 with 98% accuracy and a standard deviation of 3%. Thus, we concluded that RAPD fingerprinting of genomic DNA could be employed to discretely characterize individual mosquito of Aedes aegypti in terms of their ability to perform as a vector for Dengue virus.
KeywordsDNA fingerprinting Aedes mosquito Artificial neural network
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