Electroretinography in streptozotocin diabetic rats following acute intraocular pressure elevation
AuthorKohzaki, Kenichi; Vingrys, Algis J.; Armitage, James A.; Bui, Bang V.
Source TitleGraefe's Archive for Clinical and Experimental Ophthalmology
AffiliationDepartment of Optometry and Vision Sciences
Optometry and Vision Sciences
Document TypeJournal Article
CitationsKohzaki, K., Vingrys, A. J., Armitage, J. A. & Bui, B. V. (2013). Electroretinography in streptozotocin diabetic rats following acute intraocular pressure elevation. Graefe's Archive for Clinical and Experimental Ophthalmology, 251(2), 529-535.
Access StatusOpen Access
The research outputs in this collection have been funded in whole or in part by the National Health and Medical Research Council (NHMRC)
© Springer-Verlag Berlin Heidelberg 2012
Background: We consider whether pre-existing streptozotocin induced hyperglycemia in rats affects the ability of the eye to cope with a single episode of acute intraocular pressure (IOP) elevation. Methods: Electroretinogram (ERG) responses were measured (−6.08 to 1.92 log cd.s.m−2) in anaesthetized (60:5 mg/kg ketamine:xylazine) dark-adapted (>12 h) adult Sprague–Dawley rats 1 week after a single acute IOP elevation to 70 mmHg for 60 min. This was undertaken in rats treated 11 weeks earlier with streptozotocin (STZ, n012, 50 mg/kg at 6 weeks of age) or citrate buffer (n012). ERG responses were analyzed to derive an index of photoreceptor (a-wave), ON-bipolar (b-wave), amacrine (oscillatory potentials) and inner retinal (positive scotopic threshold response, pSTR) function. Results: One week following acute IOP elevation there was a significant reduction of the ganglion cell pSTR (−35± 11 %, P00.0161) in STZ-injected animals. In contrast the pSTR in citrate-injected animals was not significant changed (+16±14 %). The negative component of the STR was unaffected by IOP elevation in either citrate or STZ-treated groups. Photoreceptoral (a-wave, citratecontrol +4±3 %, STZ +4±5 %) and ON-bipolar cell (b-wave, control +4±3 %, STZ +4±5 %) mediated responses were not significantly affected by IOP elevation in either citrate- or STZ-injected rats. Finally, oscillatory potentials (citrate-control +8±23 %, STZ +1±17 %) were not reduced 1 week after IOP challenge. Conclusions: The ganglion cell dominated pSTR was reduced following a single episode of IOP elevation in STZ diabetic, but not control rats. These data indicate that hyperglycemia renders the inner retina more susceptible to IOP elevation.
Keywordselectroretinogram; retina; rat; streptozotocin; hyperglycemia; diabetes; intraocular pressure
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