Assessment of gene expression of intracellular calcium channels, pumps and exchangers with epidermal growth factor-induced epithelial-mesenchymal transition in a breast cancer cell line
AuthorDavis, FM; Parsonage, MT; Cabot, PJ; Parat, M-O; Thompson, EW; Roberts-Thomson, SJ; Monteith, GR
Source TitleCANCER CELL INTERNATIONAL
PublisherBIOMED CENTRAL LTD
University of Melbourne Author/sThompson, Erik
AffiliationSurgery (St Vincent's)
Document TypeJournal Article
CitationsDavis, FM; Parsonage, MT; Cabot, PJ; Parat, M-O; Thompson, EW; Roberts-Thomson, SJ; Monteith, GR, Assessment of gene expression of intracellular calcium channels, pumps and exchangers with epidermal growth factor-induced epithelial-mesenchymal transition in a breast cancer cell line, CANCER CELL INTERNATIONAL, 2013, 13
Access StatusAccess this item via the Open Access location
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3733826
NHMRC Grant codeNHMRC/1027527
BACKGROUND: Epithelial-mesenchymal transition (EMT) is a process implicated in cancer metastasis that involves the conversion of epithelial cells to a more mesenchymal and invasive cell phenotype. In breast cancer cells EMT is associated with altered store-operated calcium influx and changes in calcium signalling mediated by activation of cell surface purinergic receptors. In this study, we investigated whether MDA-MB-468 breast cancer cells induced to undergo EMT exhibit changes in mRNA levels of calcium channels, pumps and exchangers located on intracellular calcium storing organelles, including the Golgi, mitochondria and endoplasmic reticulum (ER). METHODS: Epidermal growth factor (EGF) was used to induce EMT in MDA-MB-468 breast cancer cells. Serum-deprived cells were treated with EGF (50 ng/mL) for 12 h and gene expression was assessed using quantitative RT-PCR. RESULTS AND CONCLUSIONS: These data reveal no significant alterations in mRNA levels of the Golgi calcium pump secretory pathway calcium ATPases (SPCA1 and SPCA2), or the mitochondrial calcium uniporter (MCU) or Na+/Ca2+ exchanger (NCLX). However, EGF-induced EMT was associated with significant alterations in mRNA levels of specific ER calcium channels and pumps, including (sarco)-endoplasmic reticulum calcium ATPases (SERCAs), and inositol 1,4,5-trisphosphate receptor (IP3R) and ryanodine receptor (RYR) calcium channel isoforms. The most prominent change in gene expression between the epithelial and mesenchymal-like states was RYR2, which was enriched 45-fold in EGF-treated MDA-MB-468 cells. These findings indicate that EGF-induced EMT in breast cancer cells may be associated with major alterations in ER calcium homeostasis.
KeywordsBreast cancer calcium; EMT; IP3R; RYR; SERCA; SPCA; MCU; NCLX; Breast cancer calcium; EMT; IP3R; RYR; SERCA; SPCA; MCU; NCLX
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