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dc.contributor.authorKalous, Adrianna
dc.contributor.authorNangle, Matthew R.
dc.contributor.authorAnastasia, Agustin
dc.contributor.authorHempstead, Barbara L.
dc.contributor.authorKeast, Janet R.
dc.date.accessioned2014-08-19T01:25:57Z
dc.date.available2014-08-19T01:25:57Z
dc.date.issued2012-08-27
dc.identifier.issn0022-3042
dc.identifier.urihttp://hdl.handle.net/11343/41292
dc.descriptionFulltext embargoed for: 12 months post date of publication
dc.description.abstractJ. Neurochem. (2012) 122, 523536. Abstract The nerve growth factor (NGF) precursor, proNGF, is implicated in various neuropathological states. ProNGF signals apoptosis by forming a complex with the receptors p75 and sortilin, however, it can also induce neurite growth, proposed to be mediated by the receptor of mature NGF, tyrosine kinase receptor A (TrkA). The way in which these dual effects occur in adult neurons is unclear. We investigated the neurotrophic effects of proNGF on peptidergic sensory neurons isolated from adult mouse dorsal root ganglia and found that proNGF stimulated neurite extension and branching, requiring p75, sortilin and TrkA. Neurite growth rarely occurred in sortilin-expressing neurons but was commonly observed in TrkA-positive, sortilin-negative neurons that associated closely with sortilin-positive glia. ProNGF was unable to induce local trophic effects at growth cones where sortilin-positive glia was absent. We propose that in adult sensory neurons the neurotrophic response to proNGF is mediated by NGF and TrkA, and that peri-somatic glia may participate in sortilin- and p-75 dependent cleavage of proNGF. The potential ability of local glial cells to provide a targeted supply of NGF may provide an important way to promote trophic (rather than apoptotic) outcomes under conditions where regeneration or sprouting is required.
dc.publisherWILEY-BLACKWELL
dc.subjectdorsal root ganglion
dc.subjectNeurotrophin
dc.subjectnociceptor
dc.subjectp75
dc.subjectsatellite cells
dc.subjectsortilin
dc.titleNeurotrophic actions initiated by proNGF in adult sensory neurons may require peri-somatic glia to drive local cleavage to NGF
dc.typeJournal Article
dc.identifier.doi10.1111/j.1471-4159.2012.07799.x
melbourne.affiliation.departmentAnatomy and Neuroscience
melbourne.affiliation.facultyMelbourne Medical School
melbourne.affiliation.facultyMedicine, Dentistry & Health Sciences
melbourne.source.titleJOURNAL OF NEUROCHEMISTRY
melbourne.source.monthAUG
melbourne.source.volume122
melbourne.source.issue3
melbourne.identifier.nhmrc570877
melbourne.publicationid246273
melbourne.publication.chiefinvestigatorKEAST, PROF JANET RUTH Janet
melbourne.publication.chiefinvestigatoremailjanet.keast@unimelb.edu.au
melbourne.elementsidNA
melbourne.contributor.authorKeast, Janet
melbourne.internal.ingestnoteAbstract bulk upload (2017-07-24)
melbourne.accessrightsThis item is currently not available from this repository


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