Rsx is a metatherian RNA with Xist-like properties in X-chromosome inactivation
AuthorGrant, J; Mahadevaiah, SK; Khil, P; Sangrithi, MN; Royo, H; Duckworth, J; McCarrey, JR; VandeBerg, JL; Renfree, MB; Taylor, W; ...
PublisherNATURE PUBLISHING GROUP
University of Melbourne Author/sRenfree, Marilyn
Document TypeJournal Article
CitationsGrant, J; Mahadevaiah, SK; Khil, P; Sangrithi, MN; Royo, H; Duckworth, J; McCarrey, JR; VandeBerg, JL; Renfree, MB; Taylor, W; Elgar, G; Camerini-Otero, RD; Gilchrist, MJ; Turner, JMA, Rsx is a metatherian RNA with Xist-like properties in X-chromosome inactivation, NATURE, 2012, 487 (7406), pp. 254 - U1511
Access StatusAccess this item via the Open Access location
Open Access at PMChttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC3484893
NHMRC Grant codeNHMRC/1010453
The fulltext of this publication will be made publicly available after relevant embargo periods have lapsed and associated copyright clearances obtained.
In female (XX) mammals, one of the two X chromosomes is inactivated to ensure an equal dose of X-linked genes with males (XY). X-chromosome inactivation in eutherian mammals is mediated by the non-coding RNA Xist. Xist is not found in metatherians (marsupials), and how X-chromosome inactivation is initiated in these mammals has been the subject of speculation for decades. Using the marsupial Monodelphis domestica, here we identify Rsx (RNA-on-the-silent X), an RNA that has properties consistent with a role in X-chromosome inactivation. Rsx is a large, repeat-rich RNA that is expressed only in females and is transcribed from, and coats, the inactive X chromosome. In female germ cells, in which both X chromosomes are active, Rsx is silenced, linking Rsx expression to X-chromosome inactivation and reactivation. Integration of an Rsx transgene on an autosome in mouse embryonic stem cells leads to gene silencing in cis. Our findings permit comparative studies of X-chromosome inactivation in mammals and pose questions about the mechanisms by which X-chromosome inactivation is achieved in eutherians.
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