Protein histochemistry using coronaviral spike proteins: Studying binding profiles and sialic acid requirements for attachment to tissues
No Preview Available
Citations
Altmetric:
Altmetric::
Associated Records
Authors
File Licence
Editors
Date
2015-01-01
Source Title
Methods in Molecular Biology
Journal Title
Journal ISSN
Volume Title
Publisher
Springer New York
Melbourne Authors
Affiliation
Veterinary Biosciences
Document Type
Chapter
Subject Code
Citation
Wickramasinghe, I. N. A. & Verheije, M. H. (2015). Protein histochemistry using coronaviral spike proteins: Studying binding profiles and sialic acid requirements for attachment to tissues. Coronaviruses Methods and Protocols, 1282, pp.155-163. Springer New York.
Access Status
Access this item via the Open Access location
Access URL
Open Access URL
Open Access at PMC
Linked Resource URL
NHMRC Grant Code
ARC Grant Code
Description
Abstract
Protein histochemistry is a tissue-based technique that enables the analysis of viral attachment patterns as well as the identification of specific viral and host determinants involved in the first step in the infection of a host cell by a virus. Applying recombinantly expressed spike proteins of infectious bronchitis virus onto formalin-fixed tissues allows us to profile the binding characteristics of these viral attachment proteins to tissues of various avian species. In particular, sialic acid-mediated tissue binding of spike proteins can be analyzed by pretreating tissues with various neuraminidases or by blocking the binding of the viral proteins with specific lectins. Our assay is particularly convenient to elucidate critical virus-host interactions for viruses for which infection models are limited.