Protease-activated receptor 1 in the pathogenesis of cystic fibrosis
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Date
2025-01-19
Source Title
BMJ Open Respiratory Research
Publisher
BMJ
Melbourne Authors
Affiliation
Melbourne Medical School
Paediatrics (RCH)
Paediatrics (RCH)
Document Type
Journal Article
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Sarkar, S., Han, J. X., Azzopardi, K., Dhar, P., Saeed, M. A., Day, S., Ranganathan, S. & Sutton, P. (2025). Protease-activated receptor 1 in the pathogenesis of cystic fibrosis. BMJ Open Respiratory Research, 12 (1), pp.e002960-. https://doi.org/10.1136/bmjresp-2024-002960.
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Open Access
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Abstract
BACKGROUND: The most common cause of death in those with cystic fibrosis (CF) is respiratory failure due to bronchiectasis resulting from repeated cycles of respiratory infection and inflammation. Protease-activated receptor 1 (PAR1) is a cell surface receptor activated by serine proteases including neutrophil elastase, which is recognised as a potent modulator of inflammation. While PAR1 is known to play an important role in regulating inflammation, nothing is known about any potential role of this receptor in CF pathogenesis. METHODS: PAR1 (PAR1-/- ) and intestinal-corrected CFTR (Cftr-/- ) deficient mice were crossed to generate double knock-out (DKO) mutants lacking both PAR1 and CFTR, as well as matching sibling single mutant and wildtype (WT) littermate controls. Mice were weighed weekly to 15 weeks of age; then, the lungs and intestines were examined. RESULTS: Cftr-deficient mice gained body weight at a significantly slower rate than WT controls and presented with no lung inflammation, but had increased weights of their ilea and proximal colons. DKO mice (lacking both CFTR and PAR1) gained body weight at a similar rate to Cftr-/- mice but only gained weight in their proximal colons. Weight gain in the ilea of Cftr-/- but not DKO mice was associated with increased ileal levels in the pro-inflammatory cytokine interleukin (IL)-6. CONCLUSIONS: This study provides the first evidence of PAR1 contributing to the pathological effects of Cftr deficiency in the intestine and suggests a possible effect of PAR1 on the regulation of IL-6 in CF pathogenesis.