Cell-to-cell interactions revealed by cryo-tomography of a DPANN co-culture system

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Authors
Johnson, MD; Shepherd, DC; Sakai, HD; Mudaliyar, M; Pandurangan, AP; Short, FL; Veith, PD; Scott, NE; Kurosawa, N; Ghosal, D
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2024-12-01
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Nature Communications
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NATURE PORTFOLIO
Melbourne Authors
Ghosal, Debnath ORCID
 Scott, Nichollas ORCID
 Johnson, Matthew ORCID
 Arcot Anil Kumar, Manasi ORCID
 Veith, Paul ORCID
Affiliation
Melbourne Dental School
Biochemistry and Pharmacology
Microbiology and Immunology
Document Type
Journal Article
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Citation
Johnson, M. D., Shepherd, D. C., Sakai, H. D., Mudaliyar, M., Pandurangan, A. P., Short, F. L., Veith, P. D., Scott, N. E., Kurosawa, N. & Ghosal, D. (2024). Cell-to-cell interactions revealed by cryo-tomography of a DPANN co-culture system. Nature Communications, 15 (1), https://doi.org/10.1038/s41467-024-51159-2.
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Open Access
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https://hdl.handle.net/11343/358383
DOI
10.1038/s41467-024-51159-2
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Open Access at PMC
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NHMRC Grant Code
NHMRC/1196924
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ARC/FT200100270
 ARC/DP210100362
Description
Abstract
DPANN is a widespread and diverse group of archaea characterized by their small size, reduced genome, limited metabolic pathways, and symbiotic existence. Known DPANN species are predominantly obligate ectosymbionts that depend on their host for proliferation. The structural and molecular details of host recognition, host-DPANN intercellular communication, and host adaptation in response to DPANN attachment remain unknown. Here, we use electron cryotomography (cryo-ET) to show that the Microcaldus variisymbioticus ARM-1 may interact with its host, Metallosphaera javensis AS-7 through intercellular proteinaceous nanotubes. Combining cryo-ET and sub-tomogram averaging, we show the in situ architectures of host and DPANN S-layers and the structures of the nanotubes in their primed and extended states. In addition, comparative proteomics and genomic analyses identified host proteomic changes in response to DPANN attachment. These results provide insights into the structural basis of host-DPANN communication and deepen our understanding of the host ectosymbiotic relationships.
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CC BY
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https://doi.org/10.1038/s41467-024-51159-2
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Biochemistry and Pharmacology - Research Publications
Microbiology & Immunology - Research Publications
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