TY  - JOUR
AU  - Master, JS
AU  - Thouas, GA
AU  - Harvey, AJ
AU  - Sheedy, JR
AU  - Hannan, NJ
AU  - Gardner, DK
AU  - Wlodek, ME
Y2  - 2016/09/22
Y1  - 2015/05/01
SN  - 1470-1626
UR  - http://hdl.handle.net/11343/116577
AB  - Low birth weight is associated with an increased risk for adult disease development with recent studies highlighting transmission to subsequent generations. However, the mechanisms and timing of programming of disease transmission to the next generation remain unknown. The aim of this study was to examine the effects of low birth weight and advanced maternal age on second-generation preimplantation blastocysts. Uteroplacental insufficiency or sham surgery was performed in late-gestation WKY pregnant rats, giving rise to first-generation (F1) restricted (born small) and control offspring respectively. F1 control and restricted females, at 4 or 12 months of age, were naturally mated with normal males. Second-generation (F2) blastocysts from restricted females displayed reduced expression of genes related to growth compared with F2 control (P<0.05). Following 24 h culture, F2 restricted blastocysts had accelerated development, with increased total cell number, a result of increased trophectoderm cells compared with control (P<0.05). There were alterations in carbohydrate and serine utilisation in F2 restricted blastocysts and F2 restricted outgrowths from 4-month-old females respectively (P<0.05). F2 blastocysts from aged restricted females were developmentally delayed at retrieval, with reduced total cell number attributable to reduced trophectoderm number with changes in carbohydrate utilisation (P<0.05). Advanced maternal age resulted in alterations in a number of amino acids in media obtained from F2 blastocyst outgrowths (P<0.05). These findings demonstrate that growth restriction and advanced maternal age can alter F2 preimplantation embryo physiology and the subsequent offspring growth.
LA  - English
PB  - BIOSCIENTIFICA LTD
T1  - Low female birth weight and advanced maternal age programme alterations in next-generation blastocyst development
DO  - 10.1530/REP-14-0619
IS  - REPRODUCTION
VL  - 149
IS  - 5
SP  - 497-510
OP  - 1045602
OP  - 628927
L1  - /bitstream/handle/11343/116577/Master%20preimplantation%20maternal%20embryo%20manuscript%20Accepted.pdf?sequence=1&isAllowed=y
ER  -