TY  - JOUR
AU  - Gonen, OM
AU  - Moffat, BA
AU  - Kwan, P
AU  - O'Brien, TJ
AU  - Desmond, PM
AU  - Lui, E
Y2  - 2020/11/27
Y1  - 2020/09/15
SN  - 1662-4548
UR  - http://hdl.handle.net/11343/252566
AB  - Background and Purpose: Derangements in brain glutamate, glutathione, and γ-amino butyric acid (GABA) are implicated in a range of neurological disorders. Reliable methods to measure these compounds non-invasively in vivo are needed. We evaluated the reproducibility of their measurements in brain regions involved in the default mode network using quantitative MRS at 7-Tesla in healthy individuals. Methods: Ten right-handed healthy volunteers underwent 7-Tesla MRI scans on 2 separate days, not more than 2 weeks apart. On each day two scanning sessions took place, with a re-positioning break in between. High-resolution isotropic anatomical scans were acquired prior to each scan, followed by single-voxel 1H-MRS using the STEAM pulse sequence on an 8 mL midline cubic voxel, positioned over the posterior cingulate and precuneus regions. Concentrations were corrected for partial-volume effects. Results: Maximal Cramér-Rao lower bounds for glutamate, glutathione, and GABA were 2.0, 8.0, and 14.0%, respectively. Mean coefficients of variation within sessions were 5.9 ± 4.8%, 9.3 ± 7.6%, and 11.5 ± 8.8%, and between sessions were 4.6 ± 4.5%, 8.3 ± 5.7%, and 9.2 ± 8.7%, respectively. The mean (±SD) Dice's coefficient for voxel overlap was 90 ± 4% within sessions and 86 ± 7% between sessions. Conclusion: Glutamate, glutathione, and GABA can be reliably quantified using STEAM MRS at 7-Tesla from the posterior cingulate and precuneus cortices of healthy human subjects. STEAM MRS at 7-Tesla may be used to study the metabolic behavior of this important resting-state hub in various disease states.
LA  - English
PB  - FRONTIERS MEDIA SA
T1  - Reproducibility of Glutamate, Glutathione, and GABA Measurementsin vivoby Single-Voxel STEAM Magnetic Resonance Spectroscopy at 7-Tesla in Healthy Individuals
DO  - 10.3389/fnins.2020.566643
IS  - Frontiers in Neuroscience
VL  - 14
L1  - /bitstream/handle/11343/252566/fnins-14-566643.pdf?sequence=1&isAllowed=y
ER  -