TY  - JOUR
AU  - Li, Y
AU  - Zhou, H
AU  - Cheng, L
AU  - Zhao, J
AU  - Hickford, J
Y2  - 2020/12/09
Y1  - 2020/11/03
SN  - 0003-9438
UR  - http://hdl.handle.net/11343/253038
AB  - Gene markers have become useful tools for improving animal genetics and breeding since they improve the accuracy of selection for superior breeding stock. In this study, the stearoyl-CoA desaturase (Δ-9-desaturase) gene (SCD) was investigated in New Zealand pasture-grazed Holstein–Friesian × Jersey cows. Three nucleotide substitutions were identified in exon 5 of the gene (c.702A/G, c.762T/C and c.878C/T), and a single nucleotide substitution was identified in intron 5 (c.880+105A/G). The c.878C/T substitution would, if expressed, result in the amino acid substitution p.A293V. Four nucleotide substitutions (c.*1783A/G, c.*1883C/T, c.*1984G/A and c.*2066T/C/G) were identified in the 3′-untranslated region (3′-UTR), and these resulted in three nucleotide sequence variants (named a, b and c). The sequence that would encode valine (V) at position 293 of SCD was linked to 3′-UTR variant a, and the sequence that would encode alanine (A) was linked to variants b and c. The frequency of the genotypes was as follows: VV (equivalent to aa: 15.1 %), VA (equivalent to ab+ac: 50.0 %) and AA (equivalent to bb+cc+bc: 34.9 %). The cows with the V variant produced less C10:1, C12:1 and C14:1 fatty acid (FA) but more C10:0, C11:0, C14:0, C16:1 and C18:2 FA than the A variant cows (P<0.001). Effects of c.*1783A/G and c.*2066T/C/G on milk fat composition were also found for the AA cows. The presence of c was associated with decreased levels of C16:1 (P<0.001), C17:1 (P=0.001), C18:2 cis-9, trans-13 (P=0.045), C18:2 cis-9, trans-12 (P=0.018) FA and C16:1 FA index (P<0.001). The presence of b was associated with increased levels of C13:0 iso FAs (P<0.001), monounsaturated FA (MUFA; P=0.002) and C12:1 (P<0.001).
LA  - English
PB  - Copernicus Publications
T1  - Variation in the stearoyl-CoA desaturase gene (SCD) and its influence on milk fatty acid composition in late-lactation dairy cattle grazed on pasture
DO  - 10.5194/aab-63-355-2020
IS  - Archives Animal Breeding
VL  - 63
IS  - 2
SP  - 355-366
L1  - /bitstream/handle/11343/253038/PMC7648274.pdf?sequence=1&isAllowed=y
ER  -