TY - JOUR AU - Hicks, S AU - Pohl, K AU - Neeman, T AU - McNamara, H AU - Parsons, K AU - He, J-S AU - Ali, S AU - Nazir, S AU - Rowntree, L AU - Nguyen, T AU - Kedzierska, K AU - Doolan, D AU - Vinueas, C AU - Cook, M AU - Coatsworth, N AU - Myles, P AU - Kurth, F AU - Sander, L AU - Mann, G AU - Gruen, R AU - George, A AU - Gardiner, E AU - Cockburn, I AU - the SARS-CoV-2 Testing in Elective Surgery Collaborators Y2 - 2020/12/09 Y1 - 2020/09/14 UR - http://hdl.handle.net/11343/253073 AB - Estimates of seroprevalence of SARS-CoV-2 antibodies have been hampered by inadequate assay sensitivity and specificity. Using an ELISA-based approach to that combines data about IgG responses to both the Nucleocapsid and Spike-receptor binding domain antigens, we show that near-optimal sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (0 to 0.72%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay. PB - Cold Spring Harbor Laboratory T1 - A dual antigen ELISA allows the assessment of SARS-CoV-2 antibody seroprevalence in a low transmission setting DO - 10.1101/2020.09.09.20191031 L1 - /bitstream/handle/11343/253073/EMS95138-pdf.pdf?sequence=1&isAllowed=y ER -