TY  - JOUR
AU  - Zhu, L
AU  - Shahid, MA
AU  - Markham, J
AU  - Browning, GF
AU  - Noormohammadi, AH
AU  - Marenda, MS
Y2  - 2020/12/09
Y1  - 2018/02/02
SN  - 1471-2164
UR  - http://hdl.handle.net/11343/253184
AB  - Background
The bacterial pathogen Mycoplasma synoviae can cause subclinical respiratory disease, synovitis, airsacculitis and reproductive tract disease in poultry and is a major cause of economic loss worldwide. The M. synoviae strain MS-H was developed by chemical mutagenesis of an Australian isolate and has been used as a live attenuated vaccine in many countries over the past two decades. As a result it may now be the most prevalent strain of M. synoviae globally. Differentiation of the MS-H vaccine from local field strains is important for epidemiological investigations and is often required for registration of the vaccine.

Results
The complete genomic sequence of the MS-H strain was determined using a combination of Illumina and Nanopore methods and compared to WVU-1853, the M. synoviae type strain isolated in the USA 30 years before the parent strain of MS-H, and MS53, a more recent isolate from Brazil. The vaccine strain genome had a slightly larger number of pseudogenes than the two other strains and contained a unique 55 kb chromosomal inversion partially affecting a putative genomic island. Variations in gene content were also noted, including a deoxyribose-phosphate aldolase (deoC) fragment and an ATP-dependent DNA helicase gene found only in MS-H. Some of these sequences may have been acquired horizontally from other avian mycoplasma species.

Conclusions
MS-H was somewhat more similar to WVU-1853 than to MS53. The genome sequence of MS-H will enable identification of vaccine-specific genetic markers for use as diagnostic and epidemiological tools to better control M. synoviae.
LA  - English
PB  - BioMed Central
T1  - Genome analysis of Mycoplasma synoviae strain MS-H, the most common M. synoviae strain with a worldwide distribution
DO  - 10.1186/s12864-018-4501-8
IS  - BMC Genomics
VL  - 19
IS  - 1
L1  - /bitstream/handle/11343/253184/PMC5797395.pdf?sequence=1&isAllowed=y
ER  -