TY  - JOUR
AU  - McCoy, PJ
AU  - Costello, AJ
AU  - Corcoran, NM
AU  - Hovens, CM
AU  - Clarkson, MJ
Y2  - 2020/12/10
Y1  - 2019/01/01
SN  - 2215-0161
UR  - http://hdl.handle.net/11343/253387
AB  - DNA-fluorescence in situ hybridisation (DNA-FISH) allows visualisation of chromosome organisation and rearrangement. FISH probes are pools of short fluorescently labelled DNA fragments that are often produced from template plasmids that contain large genomic inserts. For effective sample penetration and target hybridisation it is critical that probe fragments are between 200 and 500bp. Production of these short probes requires significant optimisation and can be confounded access to expensive sonication equipment or inherent sequence features that influence enzymatic fragmentation or amplification. Here we demonstrate that effective FISH probes can be prepared without the need for optimisation of fragmentation using a cocktail of two the 4bp recognition sequence restriction enzymes CviQI and AluI.
LA  - English
PB  - ELSEVIER
T1  - Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation
DO  - 10.1016/j.mex.2018.11.015
IS  - MethodsX
VL  - 6
SP  - 22-33
OP  - 1047581
OP  - 1104010
L1  - /bitstream/handle/11343/253387/PMC6308247.pdf?sequence=1&isAllowed=y
ER  -