TY  - JOUR
AU  - Zhang, X
AU  - Heng, S
AU  - Pei, J
AU  - Morey, JR
AU  - McDevitt, CA
AU  - Abell, AD
Y2  - 2020/12/10
Y1  - 2018/12/01
SN  - 2079-6374
UR  - http://hdl.handle.net/11343/253823
AB  - Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been incorporated into yeast total lipid extract-based liposomes to suppress inherent cell permeability to allow the detection of extracellular Ca2+, GSH, and Zn2+, respectively. The repurposed sensors have enhanced aqueous solubility and the ability to quantitatively measure biologically relevant concentrations of Ca2+ (0.25 mM⁻1 mM), Zn2+ (6.25 µM⁻50 µM), and GSH (0.25 mM⁻1 mM) by fluorescence in aqueous media. In addition, the liposomal sensors are nontoxic to HEK293 cells and have the ability to detect exogenously added Zn2+ (1 mM), Ca2+ (1 mM), or GSH (1 mM) near cells without internalisation. This new sensing platform provides a means to repurpose a range of intracellular fluorescent sensors to specifically detect extracellular analytes, while also improving biocompatibility for overall enhanced use in a wide range of biomedical applications.
LA  - English
PB  - MDPI
T1  - A Liposomal Platform for Sensing of Extracellular Analytes Near Cells
DO  - 10.3390/bios8040117
IS  - Biosensors
VL  - 8
IS  - 4
L1  - /bitstream/handle/11343/253823/PMC6315562.pdf?sequence=1&isAllowed=y
ER  -