TY  - JOUR
AU  - Hicks, SM
AU  - Pohl, K
AU  - Neeman, T
AU  - McNamara, HA
AU  - Parsons, KM
AU  - He, J-S
AU  - Ali, SA
AU  - Nazir, S
AU  - Rowntree, LC
AU  - Nguyen, THO
AU  - Kedzierska, K
AU  - Doolan, DL
AU  - Vinuesa, CG
AU  - Cook, MC
AU  - Coatsworth, N
AU  - Myles, PS
AU  - Kurth, F
AU  - Sander, LE
AU  - Mann, GJ
AU  - Gruen, RL
AU  - George, AJ
AU  - Gardiner, EE
AU  - Cockburn, IA
Y2  - 2020/12/16
Y1  - 2021/01/01
SN  - 0022-1899
UR  - http://hdl.handle.net/11343/254463
AB  - Estimates of seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibodies have been hampered by inadequate assay sensitivity and specificity. Using an enzyme-linked immunosorbent assay-based approach that combines data about immunoglobulin G responses to both the nucleocapsid and spike receptor binding domain antigens, we show that excellent sensitivity and specificity can be achieved. We used this assay to assess the frequency of virus-specific antibodies in a cohort of elective surgery patients in Australia and estimated seroprevalence in Australia to be 0.28% (95% Confidence Interval, 0-1.15%). These data confirm the low level of transmission of SARS-CoV-2 in Australia before July 2020 and validate the specificity of our assay.
LA  - English
PB  - OXFORD UNIV PRESS INC
T1  - A Dual-Antigen Enzyme-Linked Immunosorbent Assay Allows the Assessment of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Seroprevalence in a Low-Transmission Setting
DO  - 10.1093/infdis/jiaa623
IS  - Journal of Infectious Diseases
VL  - 223
IS  - 1
SP  - 10-14
ER  -