TY  - JOUR
AU  - Moslehi, M
AU  - Ng, DCH
AU  - Bogoyevitch, MA
Y2  - 2020/12/17
Y1  - 2017/07/12
SN  - 2045-2322
UR  - http://hdl.handle.net/11343/254693
AB  - Doublecortin X (DCX), known to be essential for neuronal migration and cortical layering in the developing brain, is a 40 kDa microtubule (MT)-associated protein. DCX directly interacts with MTs via its two structured doublecortin (DC) domains, but the dynamics of this association and the possible regulatory roles played by the flanking unstructured regions remain poorly defined. Here, we employ quantitative fluorescence recovery after photobleaching (FRAP) protocols in living cells to reveal that DCX shows remarkably rapid and complete exchange within the MT network but that the removal of the C-terminal region significantly slows this exchange. We further probed how MT organization or external stimuli could additionally modulate DCX exchange dynamics. MT depolymerisation (nocodazole treatment) or stabilization (taxol treatment) further enhanced DCX exchange rates, however the exchange rates for the C-terminal truncated DCX protein were resistant to the impact of taxol-induced stabilization. Furthermore, in response to a hyperosmotic stress stimulus, DCX exchange dynamics were slowed, and again the C-terminal truncated DCX protein was resistant to the stimulus. Thus, the DCX dynamically associates with MTs in living cells and its C-terminal region plays important roles in the MT-DCX association.
LA  - English
PB  - NATURE PUBLISHING GROUP
T1  - Dynamic microtubule association of Doublecortin X (DCX) is regulated by its C-terminus
DO  - 10.1038/s41598-017-05340-x
IS  - Scientific Reports
VL  - 7
IS  - 1
OP  - FT120100193
L1  - /bitstream/handle/11343/254693/PMC5507856.pdf?sequence=1&isAllowed=y
ER  -