TY  - JOUR
AU  - Cuiv, PO
AU  - Giri, R
AU  - Hoedt, EC
AU  - McGuckin, MA
AU  - Begun, J
AU  - Morrison, M
Y2  - 2020/12/17
Y1  - 2018/04/18
SN  - 1664-3224
UR  - http://hdl.handle.net/11343/254994
AB  - Enterococcus faecalis is an early coloniser of the human infant gut and contributes to the development of intestinal immunity. To better understand the functional capacity of E. faecalis, we constructed a broad host range RP4 mobilizable vector, pEHR513112, that confers chloramphenicol resistance and used a metaparental mating approach to isolate E. faecalis AHG0090 from a fecal sample collected from a healthy human infant. We demonstrated that E. faecalis AHG0090 is genetically tractable and could be manipulated using traditional molecular microbiology approaches. E. faecalis AHG0090 was comparable to the gold-standard anti-inflammatory bacterium Faecalibacterium prausnitzii A2-165 in its ability to suppress cytokine-mediated nuclear factor kappa B (NF-κB) activation in human gut-derived LS174T goblet cell like and Caco-2 enterocyte-like cell lines. E. faecalis AHG0090 and F. prausnitzii A2-165 produced secreted low molecular weight NF-κB suppressive peptidic bioactives. Both bioactives were sensitive to heat and proteinase K treatments although the E. faecalis AHG0090 bioactive was more resilient to both forms of treatment. As expected, E. faecalis AHG0090 suppressed IL-1β-induced NF-κB-p65 subunit nuclear translocation and expression of the NF-κB regulated genes IL-6, IL-8 and CXCL-10. Finally, we determined that E. faecalis AHG0090 is distantly related to other commensal strains and likely encodes niche factors that support effective colonization of the infant gut.
LA  - English
PB  - FRONTIERS MEDIA SA
T1  - &ITEnterococcus faecalis&IT AHG0090 is a Genetically Tractable Bacterium and Produces a Secreted Peptidic Bioactive that Suppresses Nuclear Factor Kappa B Activation in Human Gut Epithelial Cells
DO  - 10.3389/fimmu.2018.00790
IS  - Frontiers in Immunology
VL  - 9
IS  - APR
L1  - /bitstream/handle/11343/254994/PMC5915459.pdf?sequence=1&isAllowed=y
ER  -