TY  - JOUR
AU  - Jud, C
AU  - Ahmed, S
AU  - Mueller, L
AU  - Kinnear, C
AU  - Vanhecke, D
AU  - Umehara, Y
AU  - Frey, S
AU  - Liley, M
AU  - Angeloni, S
AU  - Petri-Fink, A
AU  - Rothen-Rutishauser, B
Y2  - 2020/12/18
Y1  - 2015/12/01
SN  - 2164-7860
UR  - http://hdl.handle.net/11343/255440
AB  - Epithelial tissue serves as an interface between biological compartments. Many in vitro epithelial cell models have been developed as an alternative to animal experiments to answer a range of research questions. These in vitro models are grown on permeable two-chamber systems; however, commercially available, polymer-based cell culture inserts are around 10 μm thick. Since the basement membrane found in biological systems is usually less than 1 μm thick, the 10-fold thickness of cell culture inserts is a major limitation in the establishment of realistic models. In this work, an alternative insert, accommodating an ultrathin ceramic membrane with a thickness of only 500 nm (i.e., the Silicon nitride Microporous Permeable Insert [SIMPLI]-well), was produced and used to refine an established human alveolar barrier coculture model by both replacing the conventional inserts with the SIMPLI-well and completing it with endothelial cells. The structural-functional relationship of the model was evaluated, including the translocation of gold nanoparticles across the barrier, revealing a higher translocation if compared to corresponding polyethylene terephthalate (PET) membranes. This study demonstrates the power of the SIMPLI-well system as a scaffold for epithelial tissue cell models on a truly biomimetic scale, allowing construction of more functionally accurate models of human biological barriers.
LA  - English
PB  - MARY ANN LIEBERT, INC
T1  - Ultrathin Ceramic Membranes as Scaffolds for Functional Cell Coculture Models on a Biomimetic Scale
DO  - 10.1089/biores.2015.0037
IS  - BioResearch Open Access
VL  - 4
IS  - 1
SP  - 457-468
L1  - /bitstream/handle/11343/255440/PMC4691652.pdf?sequence=1&isAllowed=y
ER  -