TY  - JOUR
AU  - ROLINK, A
AU  - GRAWUNDER, U
AU  - HAASNER, D
AU  - STRASSER, A
AU  - MELCHERS, F
Y2  - 2020/12/21
Y1  - 1993/10/01
SN  - 0022-1007
UR  - http://hdl.handle.net/11343/256358
AB  - Pro and pre B cells possess the long-term capacity to proliferate in vitro on stromal cells and interleukin 7 (IL-7) and can differentiate to surface immunoglobulin (sIg+) cells upon removal of IL-7 from the cultures. A key event in this differentiation is the extensive cell loss due to apoptosis. Because the proto-oncogene bcl-2 can promote cell survival, we established pre-B cell lines from E mu-bcl-2 transgenic mice. These pre-B cells have the same properties as those derived from non-bcl-2 transgenic mice except that they do not die by apoptosis. This allowed us to study the fate of newly formed B cells in vitro for a longer period of time. Here we show that early during the differentiation of pre-B cells, upregulation of RAG-1 and RAG-2 expression go hand in hand with rearrangements of the Ig gene loci. Moreover, the newly formed sIg+ B cells continue to express RAG-1 and RAG-2 and continue to rearrange L chain gene loci, even in the absence of proliferation, in an orderly fashion, so that kappa L+ sIg+ cells can become lambda L+ sIg+ or sIg- cells, whereas lambda L+ sIg+ cells can become sIg-, but not kappa L+ sIg+ cells. Thus, deposition of a complete Ig molecule on the surface of a B cell does not automatically stop the Ig-rearrangement machinery.
LA  - English
PB  - ROCKEFELLER UNIV PRESS
T1  - IMMATURE SURFACE IG+ B-CELLS CAN CONTINUE TO REARRANGE KAPPA-L-CHAIN AND LAMBDA-L-CHAIN GENE LOCI
DO  - 10.1084/jem.178.4.1263
IS  - Journal of Experimental Medicine
VL  - 178
IS  - 4
SP  - 1263-1270
L1  - /bitstream/handle/11343/256358/PMC2191189.pdf?sequence=1&isAllowed=y
ER  -