TY  - JOUR
AU  - Ekeberg, T
AU  - Svenda, M
AU  - Seibert, MM
AU  - Abergel, C
AU  - Maia, FRNC
AU  - Seltzer, V
AU  - DePonte, DP
AU  - Aquila, A
AU  - Andreasson, J
AU  - Iwan, B
AU  - Jonsson, O
AU  - Westphal, D
AU  - Odic, D
AU  - Andersson, I
AU  - Barty, A
AU  - Liang, M
AU  - Martin, AV
AU  - Gumprecht, L
AU  - Fleckenstein, H
AU  - Bajt, S
AU  - Barthelmess, M
AU  - Coppola, N
AU  - Claverie, J-M
AU  - Loh, ND
AU  - Bostedt, C
AU  - Bozek, JD
AU  - Krzywinski, J
AU  - Messerschmidt, M
AU  - Bogan, MJ
AU  - Hampton, CY
AU  - Sierra, RG
AU  - Frank, M
AU  - Shoeman, RL
AU  - Lomb, L
AU  - Foucar, L
AU  - Epp, SW
AU  - Rolles, D
AU  - Rudenko, A
AU  - Hartmann, R
AU  - Hartmann, A
AU  - Kimmel, N
AU  - Holl, P
AU  - Weidenspointner, G
AU  - Rudek, B
AU  - Erk, B
AU  - Kassemeyer, S
AU  - Schlichting, I
AU  - Strueder, L
AU  - Ullrich, J
AU  - Schmidt, C
AU  - Krasniqi, F
AU  - Hauser, G
AU  - Reich, C
AU  - Soltau, H
AU  - Schorb, S
AU  - Hirsemann, H
AU  - Wunderer, C
AU  - Graafsma, H
AU  - Chapman, H
AU  - Hajdu, J
Y2  - 2020/12/21
Y1  - 2016/08/01
SN  - 2052-4463
UR  - http://hdl.handle.net/11343/256407
AB  - Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limitation from radiation damage. Thus, a major part of the scientific case for the first FELs was three-dimensional (3D) reconstruction of non-crystalline biological objects. In a recent publication we demonstrated the first 3D reconstruction of a biological object from an X-ray FEL using this technique. The sample was the giant Mimivirus, which is one of the largest known viruses with a diameter of 450 nm. Here we present the dataset used for this successful reconstruction. Data-analysis methods for single-particle imaging at FELs are undergoing heavy development but data collection relies on very limited time available through a highly competitive proposal process. This dataset provides experimental data to the entire community and could boost algorithm development and provide a benchmark dataset for new algorithms.
LA  - English
PB  - NATURE PUBLISHING GROUP
T1  - Single-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
DO  - 10.1038/sdata.2016.60
IS  - Scientific Data
VL  - 3
IS  - 1
L1  - /bitstream/handle/11343/256407/PMC4968188.pdf?sequence=1&isAllowed=y
ER  -