TY - JOUR AU - Loi, M AU - Cenni, V AU - Duchi, S AU - Squarzoni, S AU - Lopez-Otin, C AU - Foisner, R AU - Lattanzi, G AU - Capanni, C Y2 - 2020/12/21 Y1 - 2016/03/29 SN - 1949-2553 UR - http://hdl.handle.net/11343/256535 AB - Chromatin disorganization is one of the major alterations linked to prelamin A processing impairment. In this study we demonstrate that BAF is necessary to modulate prelamin A effects on chromatin structure. We show that when prelamin A and BAF cannot properly interact no prelamin A-dependent effects on chromatin occur; similar to what is observed in human Nestor Guillermo Progeria Syndrome cells harboring a BAF mutation, in HEK293 cells expressing a BAF mutant unable to bind prelamin A, or in siRNA mediated BAF-depleted HEK293 cells expressing prelamin A. BAF is necessary to induce histone trimethyl-H3K9 as well as HP1-alpha and LAP2-alpha nuclear relocalization in response to prelamin A accumulation. These findings are enforced by electron microscopy evaluations showing how the prelamin A-BAF interaction governs overall chromatin organization. Finally, we demonstrate that the LAP2-alpha nuclear localization defect observed in HGPS cells involves the progerin-BAF interaction, thus establishing a functional link between BAF and prelamin A pathological forms. LA - English PB - IMPACT JOURNALS LLC T1 - Barrier-to-Autointegration Factor (BAF) involvement in prelamin A-related chromatin organization changes DO - 10.18632/oncotarget.6697 IS - Oncotarget VL - 7 IS - 13 SP - 15662-15677 L1 - /bitstream/handle/11343/256535/PMC4941268.pdf?sequence=1&isAllowed=y ER -