TY - JOUR AU - Skelton, RJP AU - Brady, B AU - Khoja, S AU - Sahoo, D AU - Engel, J AU - Arasaratnam, D AU - Saleh, KK AU - Abilez, OJ AU - Zhao, P AU - Stanley, EG AU - Elefanty, AG AU - Kwon, M AU - Elliott, DA AU - Ardehali, R Y2 - 2020/12/21 Y1 - 2016/01/12 SN - 2213-6711 UR - http://hdl.handle.net/11343/256756 AB - The generation of tissue-specific cell types from human embryonic stem cells (hESCs) is critical for the development of future stem cell-based regenerative therapies. Here, we identify CD13 and ROR2 as cell-surface markers capable of selecting early cardiac mesoderm emerging during hESC differentiation. We demonstrate that the CD13+/ROR2+ population encompasses pre-cardiac mesoderm, which efficiently differentiates to all major cardiovascular lineages. We determined the engraftment potential of CD13+/ROR2+ in small (murine) and large (porcine) animal models, and demonstrated that CD13+/ROR2+ progenitors have the capacity to differentiate toward cardiomyocytes, fibroblasts, smooth muscle, and endothelial cells in vivo. Collectively, our data show that CD13 and ROR2 identify a cardiac lineage precursor pool that is capable of successful engraftment into the porcine heart. These markers represent valuable tools for further dissection of early human cardiac differentiation, and will enable a detailed assessment of human pluripotent stem cell-derived cardiac lineage cells for potential clinical applications. LA - English PB - CELL PRESS T1 - CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells DO - 10.1016/j.stemcr.2015.11.006 IS - Stem Cell Reports VL - 6 IS - 1 SP - 95-108 L1 - /bitstream/handle/11343/256756/PMC4720015.pdf?sequence=1&isAllowed=y ER -