TY  - JOUR
AU  - Skelton, RJP
AU  - Brady, B
AU  - Khoja, S
AU  - Sahoo, D
AU  - Engel, J
AU  - Arasaratnam, D
AU  - Saleh, KK
AU  - Abilez, OJ
AU  - Zhao, P
AU  - Stanley, EG
AU  - Elefanty, AG
AU  - Kwon, M
AU  - Elliott, DA
AU  - Ardehali, R
Y2  - 2020/12/21
Y1  - 2016/01/12
SN  - 2213-6711
UR  - http://hdl.handle.net/11343/256756
AB  - The generation of tissue-specific cell types from human embryonic stem cells (hESCs) is critical for the development of future stem cell-based regenerative therapies. Here, we identify CD13 and ROR2 as cell-surface markers capable of selecting early cardiac mesoderm emerging during hESC differentiation. We demonstrate that the CD13+/ROR2+ population encompasses pre-cardiac mesoderm, which efficiently differentiates to all major cardiovascular lineages. We determined the engraftment potential of CD13+/ROR2+ in small (murine) and large (porcine) animal models, and demonstrated that CD13+/ROR2+ progenitors have the capacity to differentiate toward cardiomyocytes, fibroblasts, smooth muscle, and endothelial cells in vivo. Collectively, our data show that CD13 and ROR2 identify a cardiac lineage precursor pool that is capable of successful engraftment into the porcine heart. These markers represent valuable tools for further dissection of early human cardiac differentiation, and will enable a detailed assessment of human pluripotent stem cell-derived cardiac lineage cells for potential clinical applications.
LA  - English
PB  - CELL PRESS
T1  - CD13 and ROR2 Permit Isolation of Highly Enriched Cardiac Mesoderm from Differentiating Human Embryonic Stem Cells
DO  - 10.1016/j.stemcr.2015.11.006
IS  - Stem Cell Reports
VL  - 6
IS  - 1
SP  - 95-108
L1  - /bitstream/handle/11343/256756/PMC4720015.pdf?sequence=1&isAllowed=y
ER  -