TY  - JOUR
AU  - He, Y
AU  - Francis, F
AU  - Myers, KA
AU  - Yu, W
AU  - Black, MM
AU  - Baas, PW
Y2  - 2020/12/21
Y1  - 2005/02/28
SN  - 0021-9525
UR  - http://hdl.handle.net/11343/257184
AB  - Recent studies have shown that the transport of microtubules (MTs) and neurofilaments (NFs) within the axon is rapid, infrequent, asynchronous, and bidirectional. Here, we used RNA interference to investigate the role of cytoplasmic dynein in powering these transport events. To reveal transport of MTs and NFs, we expressed EGFP-tagged tubulin or NF proteins in cultured rat sympathetic neurons and performed live-cell imaging of the fluorescent cytoskeletal elements in photobleached regions of the axon. The occurrence of anterograde MT and retrograde NF movements was significantly diminished in neurons that had been depleted of dynein heavy chain, whereas the occurrence of retrograde MT and anterograde NF movements was unaffected. These results support a cargo model for NF transport and a sliding filament model for MT transport.
LA  - eng
PB  - Rockefeller University Press
T1  - Role of cytoplasmic dynein in the axonal transport of microtubules and neurofilaments.
DO  - 10.1083/jcb.200407191
IS  - The Journal of Cell Biology
VL  - 168
IS  - 5
SP  - 697-703
L1  - /bitstream/handle/11343/257184/PMC2171826.pdf?sequence=1&isAllowed=y
ER  -