TY  - JOUR
AU  - Cornall, AM
AU  - Poljak, M
AU  - Garland, SM
AU  - Phillips, S
AU  - Machalek, DA
AU  - Tan, JH
AU  - Quinn, MA
AU  - Tabrizi, SN
Y2  - 2020/12/21
Y1  - 2017/12/01
SN  - 2405-8521
UR  - http://hdl.handle.net/11343/257496
AB  - PURPOSE: To compare human papillomavirus genotype-specific performance of two genotyping assays, Anyplex II HPV28 (Seegene) and EuroArray HPV (EuroImmun), with Linear Array HPV (Roche). METHODS: DNA extracted from clinican-collected cervical brush specimens in PreservCyt medium (Hologic), from 403 women undergoing management for detected cytological abnormalities, was tested on the three assays. Genotype-specific agreement were assessed by Cohen's kappa statistic and Fisher's z-test of significance between proportions. RESULTS: Agreement between Linear Array and the other 2 assays was substantial to almost perfect (κ = 0.60 - 1.00) for most genotypes, and was almost perfect (κ = 0.81 - 0.98) for almost all high-risk genotypes. Linear Array overall detected most genotypes more frequently, however this was only statistically significant for HPV51 (EuroArray; p = 0.0497), HPV52 (Anyplex II; p = 0.039) and HPV61 (Anyplex II; p=0.047). EuroArray detected signficantly more HPV26 (p = 0.002) and Anyplex II detected more HPV42 (p = 0.035) than Linear Array. Each assay performed differently for HPV68 detection: EuroArray and LA were in moderate to substantial agreement with Anyplex II (κ = 0.46 and 0.62, respectively), but were in poor disagreement with each other (κ = -0.01). CONCLUSIONS: EuroArray and Anyplex II had similar sensitivity to Linear Array for most high-risk genotypes, with slightly lower sensitivity for HPV 51 or 52.
LA  - English
PB  - ELSEVIER SCIENCE BV
T1  - HPV genotype-specific concordance between EuroArray HPV, Anyplex II HPV28 and Linear Array HPV Genotyping test in Australian cervical samples
DO  - 10.1016/j.pvr.2017.10.002
IS  - Papillomavirus Research
VL  - 4
SP  - 79-84
L1  - /bitstream/handle/11343/257496/PMC5883236.pdf?sequence=1&isAllowed=y
ER  -