TY  - JOUR
AU  - Higa, Y
AU  - Uemura, R
AU  - Yamazaki, W
AU  - Goto, S
AU  - Goto, Y
AU  - Sueyoshi, M
Y2  - 2020/12/21
Y1  - 2016/08/01
SN  - 0916-7250
UR  - http://hdl.handle.net/11343/257563
AB  - We improved a loop-mediated isothermal amplification (LAMP) assay permitting sensitive and rapid Mycoplasma bovis detection. A total of 55 bacterial strains were examined in this study, including 33 M. bovis strains, 14 non-M. bovis mycoplasmas and eight non-mycoplasma bacterial strains. M. bovis was successfully detected by the LAMP assay within 60 min without cross-reaction to any other bacteria. Furthermore, a total of 135 nasal swab samples were tested directly using our LAMP assays, the previously reported LAMP assay, conventional PCR assay without pre-culture and comparing standard culture methods. The improved LAMP assay showed sensitivity and specificity of 97.2% and 90.9%, respectively (with a kappa coefficient of 0.8231), and the sensitivity of our revised LAMP assay was increased compared to existing methods.
LA  - English
PB  - JAPAN SOC VET SCI
T1  - An improved loop-mediated isothermal amplification assay for the detection of Mycoplasma bovis
DO  - 10.1292/jvms.15-0459
IS  - Journal of Veterinary Medical Science
VL  - 78
IS  - 8
SP  - 1343-1346
L1  - /bitstream/handle/11343/257563/PMC5053939.pdf?sequence=1&isAllowed=y
ER  -