TY  - JOUR
AU  - Hoe, E
AU  - Nathanielsz, J
AU  - Toh, ZQ
AU  - Spry, L
AU  - Marimla, R
AU  - Balloch, A
AU  - Mulholland, K
AU  - Licciardi, PV
Y2  - 2020/12/21
Y1  - 2016/12/01
SN  - 2072-6643
UR  - http://hdl.handle.net/11343/257571
AB  - Vitamin D induces a diverse range of biological effects, including important functions in bone health, calcium homeostasis and, more recently, on immune function. The role of vitamin D during infection is of particular interest given data from epidemiological studies suggesting that vitamin D deficiency is associated with an increased risk of infection. Vitamin D has diverse immunomodulatory functions, although its role during bacterial infection remains unclear. In this study, we examined the effects of 1,25(OH)₂D₃, the active metabolite of vitamin D, on peripheral blood mononuclear cells (PBMCs) and purified immune cell subsets isolated from healthy adults following stimulation with the bacterial ligands heat-killed pneumococcal serotype 19F (HK19F) and lipopolysaccharide (LPS). We found that 1,25(OH)₂D₃ significantly reduced pro-inflammatory cytokines TNF-α, IFN-γ, and IL-1β as well as the chemokine IL-8 for both ligands (three- to 53-fold), while anti-inflammatory IL-10 was increased (two-fold, p = 0.016) in HK19F-stimulated monocytes. Levels of HK19F-specific IFN-γ were significantly higher (11.7-fold, p = 0.038) in vitamin D-insufficient adults (<50 nmol/L) compared to sufficient adults (>50 nmol/L). Vitamin D also shifted the pro-inflammatory/anti-inflammatory balance towards an anti-inflammatory phenotype and increased the CD14 expression on monocytes (p = 0.008) in response to LPS but not HK19F stimulation. These results suggest that 1,25(OH)₂D₃ may be an important regulator of the inflammatory response and supports further in vivo and clinical studies to confirm the potential benefits of vitamin D in this context.
LA  - English
PB  - MDPI
T1  - Anti-Inflammatory Effects of Vitamin D on Human Immune Cells in the Context of Bacterial Infection
DO  - 10.3390/nu8120806
IS  - Nutrients
VL  - 8
IS  - 12
L1  - /bitstream/handle/11343/257571/PMC5188461.pdf?sequence=1&isAllowed=y
ER  -