TY  - JOUR
AU  - Tang, FSM
AU  - Van Ly, D
AU  - Spann, K
AU  - Reading, PC
AU  - Burgess, JK
AU  - Hartl, D
AU  - Baines, KJ
AU  - Oliver, BG
Y2  - 2020/12/22
Y1  - 2016/01/01
SN  - 1323-7799
UR  - http://hdl.handle.net/11343/258076
AB  - BACKGROUND AND OBJECTIVE: Respiratory viral infections are a major cause of asthma exacerbations. Neutrophils accumulate in the airways and the mechanisms that link neutrophilic inflammation, viral infections and exacerbations are unclear. This study aims to investigate anti-viral responses in neutrophils from patients with and without asthma and to investigate if neutrophils can be directly activated by respiratory viruses. METHODS: Neutrophils from peripheral blood from asthmatic and non-asthmatic individuals were isolated and stimulated with lipopolysaccharide (LPS) (1 μg/mL), f-met-leu-phe (fMLP) (100 nM), imiquimod (3 μg/mL), R848 (1.5 μg/mL), poly I:C (10 μg/mL), RV16 (multiplicity of infection (MOI)1), respiratory syncytial virus (RSV) (MOI1) or influenza virus (MOI1). Cell-free supernatants were collected after 1 h of neutrophil elastase (NE) and matrix metalloproteinase (MMP)-9 release, or after 24 h for CXCL8 release. RESULTS: LPS, fMLP, imiquimod and R848 stimulated the release of CXCL8, NE and MMP-9 whereas poly I:C selectively induced CXCL8 release only. R848-induced CXCL8 release was enhanced in neutrophils from asthmatics compared with non-asthmatic cells (P < 0.01). RSV triggered the release of CXCL8 and NE from neutrophils, whereas RV16 or influenza had no effect. CONCLUSION: Neutrophils release CXCL8, NE and MMP-9 in response to viral surrogates with R848-induced CXCL8 release being specifically enhanced in asthmatic neutrophils. Toll-like receptor (TLR7/8) dysregulation may play a role in neutrophilic inflammation in viral-induced exacerbations.
LA  - English
PB  - WILEY
T1  - Differential neutrophil activation in viral infections: Enhanced TLR-7/8-mediated CXCL8 release in asthma
DO  - 10.1111/resp.12657
IS  - Respirology
VL  - 21
IS  - 1
SP  - 172-179
L1  - /bitstream/handle/11343/258076/PMC5324549.pdf?sequence=1&isAllowed=y
ER  -